Literature DB >> 7517951

Modified technique for efficient detection of microsporidia.

E Kokoskin1, T W Gyorkos, A Camus, L Cedilotte, T Purtill, B Ward.   

Abstract

Changes in temperature (from room temperature to 50 degrees C) and staining time (from 90 to 10 min) were evaluated as a means of improving the detection of microsporidia from stool specimens. A blinded and independent comparison of 50 known positive matched-specimen pairs by three technologists resulted in consistently easier microscopic detection. The background is clearer, and spores stain more intensely. Staining time is reduced by 80 min.

Mesh：

Year:  1994        PMID： 7517951      PMCID： PMC267187          DOI： 10.1128/jcm.32.4.1074-1075.1994

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  5 in total

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Authors:  J M Orenstein
Journal:  J Parasitol       Date:  1991-12       Impact factor: 1.276

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Journal:  Can Commun Dis Rep       Date:  1993-01-30

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Authors:  J Yang; T Scholten
Journal:  Am J Clin Pathol       Date:  1977-03       Impact factor: 2.493

4.  A new trichrome-blue stain for detection of microsporidial species in urine, stool, and nasopharyngeal specimens.

Authors:  N J Ryan; G Sutherland; K Coughlan; M Globan; J Doultree; J Marshall; R W Baird; J Pedersen; B Dwyer
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948

5.  Improved light-microscopical detection of microsporidia spores in stool and duodenal aspirates. The Enteric Opportunistic Infections Working Group.

Authors:  R Weber; R T Bryan; R L Owen; C M Wilcox; L Gorelkin; G S Visvesvara
Journal:  N Engl J Med       Date:  1992-01-16       Impact factor: 91.245
  5 in total
  37 in total

Review 1.  Laboratory identification of the microsporidia.

Authors:  Lynne S Garcia
Journal:  J Clin Microbiol       Date:  2002-06       Impact factor: 5.948

2.  Comparative evaluation of modified trichrome and Uvitex 2B stains for detection of low numbers of microsporidial spores in stool specimens.

Authors:  R Ignatius; S Henschel; O Liesenfeld; U Mansmann; W Schmidt; S Köppe; T Schneider; W Heise; U Futh; E O Riecken; H Hahn; R Ullrich
Journal:  J Clin Microbiol       Date:  1997-09       Impact factor: 5.948

3.  Prevalence of microsporidium and other intestinal parasites in children from malatya, Turkey.

Authors:  Sinan Calik; Ulku Karaman; Cemil Colak
Journal:  Indian J Microbiol       Date:  2011-01-26       Impact factor: 2.461

4.  IFN-gamma-producing dendritic cells are important for priming of gut intraepithelial lymphocyte response against intracellular parasitic infection.

Authors:  Magali M Moretto; Louis M Weiss; Crescent L Combe; Imtiaz A Khan
Journal:  J Immunol       Date:  2007-08-15       Impact factor: 5.422

5.  Clinical and microscopical features of small-intestinal microsporidiosis in patients with AIDS.

Authors:  P Caramello; G Mazzucco; M Romeo; A Ullio; G DeRosa; A Lucchini; B Forno; T Brancale; A Macor; C Preziosi
Journal:  Infection       Date:  1995 Nov-Dec       Impact factor: 3.553

6.  One-step purification of Enterocytozoon bieneusi spores from human stools by immunoaffinity expanded-bed adsorption.

Authors:  I Accoceberry; M Thellier; A Datry; I Desportes-Livage; S Biligui; M Danis; X Santarelli
Journal:  J Clin Microbiol       Date:  2001-05       Impact factor: 5.948

7.  Specific PCR assay for direct detection of intestinal microsporidia Enterocytozoon bieneusi and Encephalitozoon intestinalis in fecal specimens from human immunodeficiency virus-infected patients.

Authors:  C Ombrouck; L Ciceron; S Biligui; S Brown; P Marechal; T van Gool; A Datry; M Danis; I Desportes-Livage
Journal:  J Clin Microbiol       Date:  1997-03       Impact factor: 5.948

8.  Quantitative light microscopic detection of Enterocytozoon bieneusi in stool specimens: a longitudinal study of human immunodeficiency virus-infected microsporidiosis patients.

Authors:  J E Clarridge; S Karkhanis; L Rabeneck; B Marino; L W Foote
Journal:  J Clin Microbiol       Date:  1996-03       Impact factor: 5.948

9.  Diagnosis of intestinal microsporidiosis by examination of stool and duodenal aspirate with Weber's modified trichrome and Uvitex 2B strains.

Authors:  P C DeGirolami; C R Ezratty; G Desai; A McCullough; D Asmuth; C Wanke; M Federman
Journal:  J Clin Microbiol       Date:  1995-04       Impact factor: 5.948

10.  Diagnosis of disseminated microsporidian Encephalitozoon hellem infection by PCR-Southern analysis and successful treatment with albendazole and fumagillin.

Authors:  E S Didier; L B Rogers; A D Brush; S Wong; V Traina-Dorge; D Bertucci
Journal:  J Clin Microbiol       Date:  1996-04       Impact factor: 5.948
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