| Literature DB >> 7514139 |
M Katsuyama1, Y Sugimoto, T Namba, A Irie, M Negishi, S Narumiya, A Ichikawa.
Abstract
A functional cDNA for the human prostacyclin receptor was isolated from a cDNA library of CMK cells, a human megakaryocytic leukaemia cell line. The cDNA encodes a protein consisting of 386 amino acid residues with seven putative transmembrane domains and a deduced molecular weight of 40,956. [3H]Iloprost specifically bound to the membrane of CHO cells stably expressing the cDNA with a Kd of 3.3 nM. This binding was displaced by unlabelled prostanoids in the order of iloprost = cicaprost >> carbacyclin > prostaglandin E1 (PGE1) > STA2. PGE2, PGD2 and PGF 2 alpha did not inhibit it. Iloprost in a concentration-dependent manner increased the cAMP level and generated inositol trisphosphate in these cells, indicating that this human receptor can couple to multiple signal transduction pathways.Entities:
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Year: 1994 PMID： 7514139 DOI： 10.1016/0014-5793(94)00355-6
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124