Literature DB >> 7513310

Characterization of a linear epitope on Chlamydia trachomatis serovar L2 DnaK-like protein.

S Birkelund1, B Larsen, A Holm, A G Lundemose, G Christiansen.   

Abstract

A cytoplasmic 75-kDa immunogen from Chlamydia trachomatis serovar L2 has previously been characterized as being similar to the Escherichia coli heat shock protein DnaK. We have localized a linear epitope for one monoclonal antibody specific for C. trachomatis DnaK. By use of a recombinant DNA technique, the epitope was limited to 14 amino acids. With synthetic peptides, the epitope was further limited to eight amino acids. Six of these amino acids are conserved in bovine HSP70, which has a known three-dimensional structure. The amino acid sequence homologous to the epitope is located in a linear part of the HSP70 molecule known as connect II.

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Year:  1994        PMID： 7513310      PMCID： PMC186465          DOI： 10.1128/iai.62.5.2051-2057.1994

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  30 in total

1.  Complete nucleotide sequence of the Bacillus subtilis dnaK gene.

Authors:  M Wetzstein; J Dedio; W Schumann
Journal:  Nucleic Acids Res       Date:  1990-04-25       Impact factor: 16.971

2.  Sequence analysis of the gene encoding the Chlamydia pneumoniae DnaK protein homolog.

Authors:  J M Kornak; C C Kuo; L A Campbell
Journal:  Infect Immun       Date:  1991-02       Impact factor: 3.441

3.  Three-dimensional structure of the ATPase fragment of a 70K heat-shock cognate protein.

Authors:  K M Flaherty; C DeLuca-Flaherty; D B McKay
Journal:  Nature       Date:  1990-08-16       Impact factor: 49.962

4.  The T-lymphocyte proliferative response to synthetic peptide antigens of defined secondary structure.

Authors:  S Mouritsen; M Meldal; B Rubin; A Holm; O Werdelin
Journal:  Scand J Immunol       Date:  1989-12       Impact factor: 3.487

5.  Nucleotide sequence of a Bacillus subtilis gene homologous to the dnaK gene of Escherichia coli.

Authors:  C M Hearne; D J Ellar
Journal:  Nucleic Acids Res       Date:  1989-10-25       Impact factor: 16.971

6.  Epitope mapping with solid-phase peptides: identification of type-, subspecies-, species- and genus-reactive antibody binding domains on the major outer membrane protein of Chlamydia trachomatis.

Authors:  J W Conlan; I N Clarke; M E Ward
Journal:  Mol Microbiol       Date:  1988-09       Impact factor: 3.501

7.  Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.

Authors:  D B Smith; K S Johnson
Journal:  Gene       Date:  1988-07-15       Impact factor: 3.688

8.  The 75-kilodalton cytoplasmic Chlamydia trachomatis L2 polypeptide is a DnaK-like protein.

Authors:  S Birkelund; A G Lundemose; G Christiansen
Journal:  Infect Immun       Date:  1990-07       Impact factor: 3.441

9.  The human heat-shock protein family. Expression of a novel heat-inducible HSP70 (HSP70B') and isolation of its cDNA and genomic DNA.

Authors:  T K Leung; M Y Rajendran; C Monfries; C Hall; L Lim
Journal:  Biochem J       Date:  1990-04-01       Impact factor: 3.857

10.  A hypothetical model for the peptide binding domain of hsp70 based on the peptide binding domain of HLA.

Authors:  F Rippmann; W R Taylor; J B Rothbard; N M Green
Journal:  EMBO J       Date:  1991-05       Impact factor: 11.598
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  5 in total

1.  Chlamydial serology: comparative diagnostic value of immunoblotting, microimmunofluorescence test, and immunoassays using different recombinant proteins as antigens.

Authors:  S Bas; P Muzzin; B Ninet; J E Bornand; C Scieux; T L Vischer
Journal:  J Clin Microbiol       Date:  2001-04       Impact factor: 5.948

2.  Molecular design of Mycoplasma hominis Vaa adhesin.

Authors:  T Boesen; N U Fedosova; M Kjeldgaard; S Birkelund; G Christiansen
Journal:  Protein Sci       Date:  2001-12       Impact factor: 6.725

3.  Surface accessibility of the 70-kilodalton Chlamydia trachomatis heat shock protein following reduction of outer membrane protein disulfide bonds.

Authors:  Jane E Raulston; Carolyn H Davis; Terry R Paul; J Dave Hobbs; Priscilla B Wyrick
Journal:  Infect Immun       Date:  2002-02       Impact factor: 3.441

4.  Cloning and expression of the dnaK gene of Campylobacter jejuni and antigenicity of heat shock protein 70.

Authors:  F L Thies; H Karch; H P Hartung; G Giegerich
Journal:  Infect Immun       Date:  1999-03       Impact factor: 3.441

5.  Characterization of two conformational epitopes of the Chlamydia trachomatis serovar L2 DnaK immunogen.

Authors:  S Birkelund; P Mygind; A Holm; B Larsen; F Beck; G Christiansen
Journal:  Infect Immun       Date:  1996-03       Impact factor: 3.441
  5 in total

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