Literature DB >> 7513181

Growth of human vascular endothelial cells on various types of microcarriers.

G Schrimpf1, P Friedl.   

Abstract

Various types of microcarriers were tested as growth substrate for the cultivation of either endothelial cells from human umbilical cord veins or of EA.hy926, an immortalized cell line of endothelial origin. Cell growth was tested on microcarriers in tissue culture flasks and spinner flasks. Solid (Cytodex type I, II, III, Gelibeads, Mica) and macroporous (Polyhipe, CultiSpher GL, PolyporE type I) microcarriers were tested. For the solid carriers the best results were obtained with Mica and for the macroporous carriers with CultiSpher GL.

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Year:  1993        PMID： 7513181     DOI： 10.1007/bf00749816

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  4 in total

1.  An optimized culture medium for human vascular endothelial cells from umbilical cord veins.

Authors:  P Friedl; D Tatje; R Czpla
Journal:  Cytotechnology       Date:  1989-08       Impact factor: 2.058

2.  Fermentation of bovine endothelial cells for preparation of endothelial cell-surface heparan sulphate.

Authors:  K R Jerg; H Baumann; R Keller; P Friedl
Journal:  Int J Biol Macromol       Date:  1990-04       Impact factor: 6.953

3.  Determination of the number of endothelial cells in culture using an acid phosphatase assay.

Authors:  D T Connolly; M B Knight; N K Harakas; A J Wittwer; J Feder
Journal:  Anal Biochem       Date:  1986-01       Impact factor: 3.365

4.  Permanent cell line expressing human factor VIII-related antigen established by hybridization.

Authors:  C J Edgell; C C McDonald; J B Graham
Journal:  Proc Natl Acad Sci U S A       Date:  1983-06       Impact factor: 11.205
  4 in total
  5 in total

1.  Metabolic rates of vascular endothelial cellsin vitro.

Authors:  G Schrimpf; M Schröder; E Weitnauer; P Friedl
Journal:  Cytotechnology       Date:  1994-01       Impact factor: 2.058

2.  Scale up cultivation of primary human umbilical vein endothelial cells on microcarriers from spinner vessels to bioreactor fermentation.

Authors:  S Duvar; J Müthing; H Mohr; J Lehmann
Journal:  Cytotechnology       Date:  1996-01       Impact factor: 2.058

3.  Cell growth and protein formation on various microcarriers.

Authors:  D Kong; M Chen; R Gentz; J Zhang
Journal:  Cytotechnology       Date:  1999-03       Impact factor: 2.058

Review 4.  [Techniques for functional tissue and organ replacement using postnatal stem cells].

Authors:  J Aigner; M Eblenkamp; E Wintermantel
Journal:  Chirurg       Date:  2005-05       Impact factor: 0.955

5.  Microcarrier cultivation of bovine aortic endothelial cells in spinner vessels and a membrane stirred bioreactor.

Authors:  J Müthing; S Duvar; S Nerger; H Büntemeyer; J Lehmann
Journal:  Cytotechnology       Date:  1995-01       Impact factor: 2.058
  5 in total

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