A controlled hematoxylin-eosin for actinic elastosis-lysis. Its versatility and use for vascular damage (actinic arteriopathy) in the skin and orbit.

To demonstrate elastic tissue and its reactions in the skin and other organs, the hematoxylin-eosin staining method had been modified in two ways: (a) the hematoxylin solution is used once only to obtain its full potential, and (b) aqueous eosin is given greater selectivity by controlling its pH with a buffer. This stain highlights the broad supporting role of elastic tissue, particularly in pressure-containing blood vessels. It also helps to define the hyperplasia, degeneration, and elastolysis that arise from its peculiar sensitivity to actinic radiation.