Literature DB >> 7512732

The human splicing factor ASF/SF2 can specifically recognize pre-mRNA 5' splice sites.

P Zuo1, J L Manley.   

Abstract

ASF/SF2 is a human protein previously shown to function in in vitro pre-mRNA splicing as an essential factor necessary for all splices and also as an alternative splicing factor, capable of switching selection of 5' splice sites. To begin to study the protein's mechanism of action, we have investigated the RNA binding properties of purified recombinant ASF/SF2. Using UV crosslinking and gel shift assays, we demonstrate that the RNA binding region of ASF/SF2 can interact with RNA in a sequence-specific manner, recognizing the 5' splice site in each of two different pre-mRNAs. Point mutations in the 5' splice site consensus can reduce binding by as much as a factor of 100, with the largest effects observed in competition assays. These findings support a model in which ASF/SF2 aids in the recognition of pre-mRNA 5' splice sites.

Entities:  

Mesh：

Substances：

Year:  1994        PMID： 7512732      PMCID： PMC43577          DOI： 10.1073/pnas.91.8.3363

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  52 in total

1.  Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells.

Authors:  A R Krainer; G C Conway; D Kozak
Journal:  Genes Dev       Date:  1990-07       Impact factor: 11.361

2.  Splicing of SV40 early pre-mRNA to large T and small t mRNAs utilizes different patterns of lariat branch sites.

Authors:  J C Noble; Z Q Pan; C Prives; J L Manley
Journal:  Cell       Date:  1987-07-17       Impact factor: 41.582

3.  A compensatory base change in U1 snRNA suppresses a 5' splice site mutation.

Authors:  Y Zhuang; A M Weiner
Journal:  Cell       Date:  1986-09-12       Impact factor: 41.582

Review 4.  hnRNP proteins and the biogenesis of mRNA.

Authors:  G Dreyfuss; M J Matunis; S Piñol-Roma; C G Burd
Journal:  Annu Rev Biochem       Date:  1993       Impact factor: 23.643

5.  A protein factor, ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro.

Authors:  H Ge; J L Manley
Journal:  Cell       Date:  1990-07-13       Impact factor: 41.582

6.  mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.

Authors:  S A Adam; T Nakagawa; M S Swanson; T K Woodruff; G Dreyfuss
Journal:  Mol Cell Biol       Date:  1986-08       Impact factor: 4.272

7.  Specific commitment of different pre-mRNAs to splicing by single SR proteins.

Authors:  X D Fu
Journal:  Nature       Date:  1993-09-02       Impact factor: 49.962

8.  A splicing enhancer complex controls alternative splicing of doublesex pre-mRNA.

Authors:  M Tian; T Maniatis
Journal:  Cell       Date:  1993-07-16       Impact factor: 41.582

9.  A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: domain structure and expression.

Authors:  A B Sachs; M W Bond; R D Kornberg
Journal:  Cell       Date:  1986-06-20       Impact factor: 41.582

10.  Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for pre-mRNA splicing in vitro.

Authors:  A R Krainer; T Maniatis
Journal:  Cell       Date:  1985-10       Impact factor: 41.582
View more
  55 in total

1.  Identification of a bidirectional splicing enhancer: differential involvement of SR proteins in 5' or 3' splice site activation.

Authors:  C F Bourgeois; M Popielarz; G Hildwein; J Stevenin
Journal:  Mol Cell Biol       Date:  1999-11       Impact factor: 4.272

2.  Identification and characterization of a novel serine-arginine-rich splicing regulatory protein.

Authors:  D C Barnard; J G Patton
Journal:  Mol Cell Biol       Date:  2000-05       Impact factor: 4.272

3.  The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.

Authors:  Y Cavaloc; C F Bourgeois; L Kister; J Stévenin
Journal:  RNA       Date:  1999-03       Impact factor: 4.942

4.  An intronic splicing enhancer binds U1 snRNPs to enhance splicing and select 5' splice sites.

Authors:  A J McCullough; S M Berget
Journal:  Mol Cell Biol       Date:  2000-12       Impact factor: 4.272

Review 5.  Sorting out the complexity of SR protein functions.

Authors:  B R Graveley
Journal:  RNA       Date:  2000-09       Impact factor: 4.942

6.  Mutations in tau gene exon 10 associated with FTDP-17 alter the activity of an exonic splicing enhancer to interact with Tra2 beta.

Authors:  Zhihong Jiang; Hao Tang; Necat Havlioglu; Xiaochun Zhang; Stefan Stamm; Riqiang Yan; Jane Y Wu
Journal:  J Biol Chem       Date:  2003-03-20       Impact factor: 5.157

7.  The second RNA-binding domain of the human splicing factor ASF/SF2 is the critical domain controlling adenovirus E1A alternative 5'-splice site selection.

Authors:  Vita Dauksaite; Göran Akusjärvi
Journal:  Biochem J       Date:  2004-07-15       Impact factor: 3.857

8.  The C-terminal domain of the largest subunit of RNA polymerase II interacts with a novel set of serine/arginine-rich proteins.

Authors:  A Yuryev; M Patturajan; Y Litingtung; R V Joshi; C Gentile; M Gebara; J L Corden
Journal:  Proc Natl Acad Sci U S A       Date:  1996-07-09       Impact factor: 11.205

9.  Enhancer-dependent 5'-splice site control of fruitless pre-mRNA splicing.

Authors:  Bianca J Lam; Arati Bakshi; Fatma Y Ekinci; Jenny Webb; Brenton R Graveley; Klemens J Hertel
Journal:  J Biol Chem       Date:  2003-03-19       Impact factor: 5.157

10.  Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2.

Authors:  S H Xiao; J L Manley
Journal:  EMBO J       Date:  1998-11-02       Impact factor: 11.598
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.