Literature DB >> 7510536

DNA strandness changes during in situ hybridization conditions.

D Peretti1, R Mezzanotte.   

Abstract

DNA strandness changes occurring during in situ hybridization were monitored using monoclonal antibodies. Our results show that: 1) RNase induces formation of single stranded DNA, 2) after denaturation-renaturation, single stranded DNA is found principally in centromeric areas, and 3) double stranded DNA is still observed after each step.

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Year:  1993        PMID: 7510536

Source DB:  PubMed          Journal:  Eur J Histochem        ISSN: 1121-760X            Impact factor:   3.188


  1 in total

1.  RNA metabolism in situ at the 93D heat shock locus in polytene nuclei of Drosophila melanogaster after various treatments.

Authors:  S C Lakhotia; A Sharma
Journal:  Chromosome Res       Date:  1995-05       Impact factor: 5.239

  1 in total

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