Literature DB >> 7507107

Modulation of HIV-1 reverse transcriptase function in "selectively deleted" p66/p51 heterodimers.

P S Jacques1, B M Wöhrl, K J Howard, S F Le Grice.   

Abstract

A contribution of the 51-kDa subunit of human immunodeficiency virus type-1 reverse transcriptase to activities of the parental heterodimer (p66/p51) was assessed in "selectively deleted" heterodimers whose p51 component contained C-terminal truncations of 13, 19, or 25 residues. Analyses included (i) efficiency of reconstitution into heterodimer, (ii) retention of polymerase and ribonuclease H (RNase H) function, and (iii) interaction with the HIV replication primer, tRNA(Lys,3). Our data suggest that these features of heterodimer reverse transcriptase can be modulated by the extent of the C-terminal p51 deletion. Severely impaired tRNA binding in a selectively deleted heterodimer whose 51-kDa subunit lacks 13 residues, despite retention of enzymatic functions, strengthens arguments for p51 involvement in tRNA binding.

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Year:  1994        PMID： 7507107

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  16 in total

1.  Analysis of mutations and suppressors affecting interactions between the subunits of the HIV type 1 reverse transcriptase.

Authors:  G Tachedjian; H E Aronson; S P Goff
Journal:  Proc Natl Acad Sci U S A       Date:  2000-06-06       Impact factor: 11.205

2.  Effects of alterations of primer-binding site sequences on human immunodeficiency virus type 1 replication.

Authors:  X Li; J Mak; E J Arts; Z Gu; L Kleiman; M A Wainberg; M A Parniak
Journal:  J Virol       Date:  1994-10       Impact factor: 5.103

3.  Structural basis of asymmetry in the human immunodeficiency virus type 1 reverse transcriptase heterodimer.

Authors:  J Wang; S J Smerdon; J Jäger; L A Kohlstaedt; P A Rice; J M Friedman; T A Steitz
Journal:  Proc Natl Acad Sci U S A       Date:  1994-07-19       Impact factor: 11.205

4.  Binding of RNA template to a complex of HIV-1 reverse transcriptase/primer/template.

Authors:  B Canard; R Sarfati; C C Richardson
Journal:  Proc Natl Acad Sci U S A       Date:  1997-10-14       Impact factor: 11.205

5.  Mutations in HIV reverse transcriptase which alter RNase H activity and decrease strand transfer efficiency are suppressed by HIV nucleocapsid protein.

Authors:  C E Cameron; M Ghosh; S F Le Grice; S J Benkovic
Journal:  Proc Natl Acad Sci U S A       Date:  1997-06-24       Impact factor: 11.205

Review 6.  Human immunodeficiency virus reverse transcriptase: 25 years of research, drug discovery, and promise.

Authors:  Stuart F J Le Grice
Journal:  J Biol Chem       Date:  2012-10-05       Impact factor: 5.157

7.  Subunit-specific mutagenesis of the cysteine 280 residue of the reverse transcriptase of human immunodeficiency virus type 1: effects on sensitivity to a specific inhibitor of the RNase H activity.

Authors:  S Loya; H Q Gao; O Avidan; P L Boyer; S H Hughes; A Hizi
Journal:  J Virol       Date:  1997-07       Impact factor: 5.103

8.  Mutagenesis of human immunodeficiency virus reverse transcriptase p51 subunit defines residues contributing to vinylogous urea inhibition of ribonuclease H activity.

Authors:  Suhman Chung; Jennifer T Miller; Barry C Johnson; Stephen H Hughes; Stuart F J Le Grice
Journal:  J Biol Chem       Date:  2011-11-21       Impact factor: 5.157

9.  Subunit-specific analysis of the human immunodeficiency virus type 1 reverse transcriptase in vivo.

Authors:  Alok Mulky; Stefan G Sarafianos; Edward Arnold; Xiaoyun Wu; John C Kappes
Journal:  J Virol       Date:  2004-07       Impact factor: 5.103

10.  Examining the role of the HIV-1 reverse transcriptase p51 subunit in positioning and hydrolysis of RNA/DNA hybrids.

Authors:  Suhman Chung; Jennifer T Miller; Mikalai Lapkouski; Lan Tian; Wei Yang; Stuart F J Le Grice
Journal:  J Biol Chem       Date:  2013-04-17       Impact factor: 5.157
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