Distribution of alpha 2-macroglobulin and alpha 1-acid glycoprotein mRNA shows regional specialization in rat decidua.

In situ hybridization histochemistry was used to detect mRNA coding for the plasma proteins alpha 1-acid glycoprotein and alpha 2-macroglobulin in the rat decidua during the period when the chorioallantoic placenta is established. It was found that alpha 1-acid glycoprotein mRNA was localized to a subpopulation of decidual cells predominantly found in the decidua capsularis but extending into the decidua basalis at later times. The highest levels of alpha 2-macroglobulin mRNA were found in the decidua basalis where there was some overlap with regions containing alpha 1-acid glycoprotein mRNA. No alpha 2-macroglobulin mRNA could be found in the inner part of the decidua capsularis where the highest levels of alpha 1-acid glycoprotein mRNA were found. However, a thin outer layer of compressed stromal cells, adjacent to the myometrium expressed the alpha 2-macroglobulin gene which surrounded the cells containing alpha 1-acid glycoprotein mRNA. This distribution of alpha 2-macroglobulin mRNA is consistent with the hypothesis that the protein is produced locally to prevent non-specific proteolysis which may otherwise result from catabolic processes involved in tissue remodelling. The function of alpha 1-acid glycoprotein is unknown but this protein is also likely to be involved in the maintenance of homeostasis during the period when contact between maternal and fetal systems is being established within the chorioallantoic placenta.