| Literature DB >> 7502578 |
G Cardinali1, L Pellegrini, A Martini.
Abstract
A modified procedure is proposed for chromosomal DNA extraction based on a cell-wall lytic enzyme never applied before in pulsed field gel electrophoresis. Protoplasting efficiency is retained under very challenging conditions for enzyme activity, such as those required for non-Saccharomyces yeasts often characterized by cell walls highly resistant to lysis.Entities:
Mesh：
Substances：
Year: 1995 PMID： 7502578 DOI： 10.1002/yea.320111104
Source DB: PubMed Journal: Yeast ISSN： 0749-503X Impact factor: 3.239