Interleukin-1 (IL-1) bioactivity and inhibition of IL-1 bioactivity in supernatant fluid from cultured microphthalmic (mi) mice teeth of different ages.

A functioning dental follicle and bone resorption are necessary for tooth eruption. In the microphthalmic (mi) mouse, bone resorption is defective and teeth fail to erupt. Local bone metabolism involves the production of cytokines such as interleukin-1 alpha (IL-1 alpha) and transforming growth factor-beta (TGF-beta). The production of these cytokines by dental tissues during eruption may be fundamental to tooth movement. Molars from mi mice were cultured and supernatants tested for the presence of these cytokines using bio-dot blotting and ELISA. A thymocyte bioassay was used to test supernatants for IL-1 bioactivity and IL-1 inhibition bioactivity. IL-1 alpha and TGF-beta were detected in all supernatants. Supernatants demonstrated no IL-1 bioactivity but inhibited IL-1 bioactivity which varied with concentration of supernatant, age and animal. This study demonstrated that cultured developing teeth secrete IL-1 alpha and TGF-beta however, concentrations varied in normal and pathological states. While IL-1 alpha was present in the supernatants, all demonstrated a variable ability to inhibit IL-1 bioactivity. This ability may influence local bone metabolism and hence tooth eruption.