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Literature DB >> 7497468

Improved genomic/nuclear DNA extraction for 8-hydroxydeoxyguanosine analysis of small amounts of rat liver tissue.

D Nakae¹, Y Mizumoto, E Kobayashi, O Noguchi, Y Konishi.

Abstract

Using two different commercially available extraction kits, genomic/nuclear DNA could be recovered from rat liver samples as small as 10 mg. Background 8-hydroxydeoxyguanosine levels in such DNA were low and stable at 0.26-0.30 +/- 0.01-0.03/10(5) guanine residues. The minimum tissue wet weight required for the accurate 8-hydroxydeoxyguanosine analysis was 25 mg. The present results indicate that routine and reliable assessment of the involvement of oxidative DNA damage in the development of various diseases, including cancer, is feasible using a variety of tissue sources.

Entities: Chemical Disease Species

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Year: 1995 PMID： 7497468 DOI： 10.1016/0304-3835(95)03980-b

Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679

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Cited

24 in total

1. A reliable assessment of 8-oxo-2-deoxyguanosine levels in nuclear and mitochondrial DNA using the sodium iodide method to isolate DNA.

Authors: M L Hamilton; Z Guo; C D Fuller; H Van Remmen; W F Ward; S N Austad; D A Troyer; I Thompson; A Richardson
Journal: Nucleic Acids Res Date: 2001-05-15 Impact factor: 16.971

2. Indirect mutagenesis by oxidative DNA damage: formation of the pyrimidopurinone adduct of deoxyguanosine by base propenal.

Authors: P C Dedon; J P Plastaras; C A Rouzer; L J Marnett
Journal: Proc Natl Acad Sci U S A Date: 1998-09-15 Impact factor: 11.205

3. DNA oxidation matters: the HPLC-electrochemical detection assay of 8-oxo-deoxyguanosine and 8-oxo-guanine.

Authors: H J Helbock; K B Beckman; M K Shigenaga; P B Walter; A A Woodall; H C Yeo; B N Ames
Journal: Proc Natl Acad Sci U S A Date: 1998-01-06 Impact factor: 11.205

4. Does oxidative damage to DNA increase with age?

Authors: M L Hamilton; H Van Remmen; J A Drake; H Yang; Z M Guo; K Kewitt; C A Walter; A Richardson
Journal: Proc Natl Acad Sci U S A Date: 2001-08-21 Impact factor: 11.205

5. Site-specific radical formation in DNA induced by Cu(II)-H₂O₂ oxidizing system, using ESR, immuno-spin trapping, LC-MS, and MS/MS.

Authors: Suchandra Bhattacharjee; Leesa J Deterding; Saurabh Chatterjee; Jinjie Jiang; Marilyn Ehrenshaft; Olivier Lardinois; Dario C Ramirez; Kenneth B Tomer; Ronald P Mason
Journal: Free Radic Biol Med Date: 2011-03-05 Impact factor: 7.376

6. Contrasting genome-wide distribution of 8-hydroxyguanine and acrolein-modified adenine during oxidative stress-induced renal carcinogenesis.

Authors: Shinya Akatsuka; Than Tin Aung; Khokon Kumar Dutta; Li Jiang; Wen-Hua Lee; Yu-Ting Liu; Janice Onuki; Tomoyuki Shirase; Kyoko Yamasaki; Hirotomo Ochi; Yuji Naito; Toshikazu Yoshikawa; Hiroshi Kasai; Yohei Tominaga; Kunihiko Sakumi; Yusaku Nakabeppu; Yoshichika Kawai; Koji Uchida; Aiichi Yamasaki; Tatsuaki Tsuruyama; Yoshihiro Yamada; Shinya Toyokuni
Journal: Am J Pathol Date: 2006-10 Impact factor: 4.307

Improved genomic/nuclear DNA extraction for 8-hydroxydeoxyguanosine analysis of small amounts of rat liver tissue.

1. A reliable assessment of 8-oxo-2-deoxyguanosine levels in nuclear and mitochondrial DNA using the sodium iodide method to isolate DNA.

2. Indirect mutagenesis by oxidative DNA damage: formation of the pyrimidopurinone adduct of deoxyguanosine by base propenal.

3. DNA oxidation matters: the HPLC-electrochemical detection assay of 8-oxo-deoxyguanosine and 8-oxo-guanine.

4. Does oxidative damage to DNA increase with age?

5. Site-specific radical formation in DNA induced by Cu(II)-H₂O₂ oxidizing system, using ESR, immuno-spin trapping, LC-MS, and MS/MS.

6. Contrasting genome-wide distribution of 8-hydroxyguanine and acrolein-modified adenine during oxidative stress-induced renal carcinogenesis.

7. Suppression of lipid-hydroperoxide and DNA-adduct formation by isoflavone-containing soy hypocotyl tea in rats.

8. Reactive oxygen species in choline deficiency induced carcinogenesis and nitrone inhibition.

9. Different effects of CSA and CSB deficiency on sensitivity to oxidative DNA damage.

10. Generation of guanine-thymine cross-links in human cells by one-electron oxidation mechanisms.