Literature DB >> 7495556

Automated DNA purification and amplification from blood-stained cards using a robotic workstation.

P Belgrader1, S A Del Rio, K A Turner, M A Marino, K R Weaver, P E Williams.   

Abstract

A new method for the purification of DNA on blood-stained cards was developed. This method was implemented into a high-throughput automated system using a Biomek 1000 robotic workstation. In addition, the processes of DNA purification and amplification were coupled into a completely automated and uninterrupted prototype system, and the resultant PCR products generated by this system were subjected to automated desalting for capillary electrophoresis analysis.

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Year:  1995        PMID： 7495556

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  5 in total

1.  Extraction-free, filter-based template preparation for rapid and sensitive PCR detection of pathogenic parasitic protozoa.

Authors:  P A Orlandi; K A Lampel
Journal:  J Clin Microbiol       Date:  2000-06       Impact factor: 5.948

Review 2.  Formation of magnetite by bacteria and its application.

Authors:  Atsushi Arakaki; Hidekazu Nakazawa; Michiko Nemoto; Tetsushi Mori; Tadashi Matsunaga
Journal:  J R Soc Interface       Date:  2008-09-06       Impact factor: 4.118

3.  Improved template preparation for PCR-based assays for detection of food-borne bacterial pathogens.

Authors:  K A Lampel; P A Orlandi; L Kornegay
Journal:  Appl Environ Microbiol       Date:  2000-10       Impact factor: 4.792

4.  Detection of hemochromatosis through the analysis of single- nucleotide extension products by capillary electrophoresis.

Authors:  Q Liang; P A Davis; J T Simpson; B H Thompson; J M Devaney; J Girard
Journal:  J Biomol Tech       Date:  2000-06

5.  The best practice for preparation of samples from FTA®cards for diagnosis of blood borne infections using African trypanosomes as a model system.

Authors:  Heba A Ahmed; Ewan T MacLeod; Geoff Hide; Susan C Welburn; Kim Picozzi
Journal:  Parasit Vectors       Date:  2011-05-07       Impact factor: 3.876
  5 in total

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