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Literature DB >> 7494051

A simple and sensitive DNA hybridization assay used for the routine diagnosis of human parvovirus B19 infection.

K E Hicks¹, S Beard, B J Cohen, J P Clewley.

Abstract

A dot blot hybridization assay for parvovirus B19 diagnosis was developed by using a PCR-generated probe, digoxigenin labelling, and chemiluminescence detection. Different labelling techniques and hybridization solutions were evaluated. From this analysis a protocol was devised for routine diagnostic use. The protocol enabled 1 pg of B19 DNA to be detected. The results of applying this method to 8,369 diagnostic samples collected during 1994 and 1995 are given.

Entities: Chemical Disease Gene Species

Mesh：

Substances：
DNA, Viral

Year: 1995 PMID： 7494051 PMCID： PMC228444 DOI： 10.1128/jcm.33.9.2473-2475.1995

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

20 in total

1. Molecular approaches for production of B19 antigen.

Authors: J P Clewley; J Mori; J Turton
Journal: Behring Inst Mitt Date: 1990-08

2. Persistent B19 parvovirus infection as a cause of severe chronic anaemia in children with acute lymphocytic leukaemia.

Authors: G J Kurtzman; B Cohen; P Meyers; A Amunullah; N S Young
Journal: Lancet Date: 1988-11-19 Impact factor: 79.321

3. Rapid screening for B19 parvovirus DNA in clinical specimens with a digoxigenin-labeled DNA hybridization probe.

Authors: M Zerbini; M Musiani; S Venturoli; G Gallinella; D Gibellini; G Gentilomi; M La Placa
Journal: J Clin Microbiol Date: 1990-11 Impact factor: 5.948

4. Parvovirus infections and hypoplastic crisis in sickle-cell anaemia.

Authors: J R Pattison; S E Jones; J Hodgson; L R Davis; J M White; C E Stroud; L Murtaza
Journal: Lancet Date: 1981-03-21 Impact factor: 79.321

5. Persistent B19 parvovirus infection in patients infected with human immunodeficiency virus type 1 (HIV-1): a treatable cause of anemia in AIDS.

Authors: N Frickhofen; J L Abkowitz; M Safford; J M Berry; J Antunez-de-Mayolo; A Astrow; R Cohen; I Halperin; L King; D Mintzer
Journal: Ann Intern Med Date: 1990-12-15 Impact factor: 25.391

A simple and sensitive DNA hybridization assay used for the routine diagnosis of human parvovirus B19 infection.

1. Molecular approaches for production of B19 antigen.

2. Persistent B19 parvovirus infection as a cause of severe chronic anaemia in children with acute lymphocytic leukaemia.

3. Rapid screening for B19 parvovirus DNA in clinical specimens with a digoxigenin-labeled DNA hybridization probe.

4. Parvovirus infections and hypoplastic crisis in sickle-cell anaemia.

5. Persistent B19 parvovirus infection in patients infected with human immunodeficiency virus type 1 (HIV-1): a treatable cause of anemia in AIDS.

6. Dot blot hybridization assay of B19 virus DNA in clinical specimens.

7. Production of digoxigenin-labelled parvovirus DNA probe by PCR.

8. Parvovirus B19 outbreak on an adult ward.

9. Rapid detection of PCR products of hepatitis C virus (HCV) with a non-radioactive oligoprobe.

10. Long-term outcome in fetal hydrops from parvovirus B19 infection.

1. Sequence analysis of a parvovirus B19 isolate and baculovirus expression of the non-structural protein.

2. Dot immunoperoxidase assay for detection of parvovirus B19 antigens in serum samples.

3. Detection of parvovirus B19 DNA in bone marrow cells by chemiluminescence in situ hybridization.