Literature DB >> 7489909

New multifunctional Escherichia coli-Streptomyces shuttle vectors allowing blue-white screening on XGal plates.

U F Wehmeier1.   

Abstract

Four new shuttle vectors for Escherichia coli (Ec) and Streptomyces, pUWL218, pUWL219, pUWL-SK and pUWL-KS, which permit recognition of recombinant (re-) plasmids on XGal plates in Ec, were constructed. These vectors contain the replication functions of the Streptomyces wide-host-range multicopy plasmid pIJ101, the tsr gene conferring resistance to thiostrepton in Streptomyces, the ColEI origin of replication from the pUC plasmids for replication in Ec and the bla gene conferring resistance to ampicillin in Ec. They possess multiple cloning sites with a number of unique restriction sites and allow direct sequencing of re-derivatives using the pUC sequencing primers.

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Year:  1995        PMID： 7489909     DOI： 10.1016/0378-1119(95)00513-6

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  13 in total

1.  Identification by gene deletion analysis of a regulator, VmsR, that controls virginiamycin biosynthesis in Streptomyces virginiae.

Authors:  R Kawachi; U Wangchaisoonthorn; T Nihira; Y Yamada
Journal:  J Bacteriol       Date:  2000-11       Impact factor: 3.490

2.  Biosynthetic pathway for mannopeptimycins, lipoglycopeptide antibiotics active against drug-resistant gram-positive pathogens.

Authors:  Nathan A Magarvey; Brad Haltli; Min He; Michael Greenstein; John A Hucul
Journal:  Antimicrob Agents Chemother       Date:  2006-06       Impact factor: 5.191

3.  FtsW is a dispensable cell division protein required for Z-ring stabilization during sporulation septation in Streptomyces coelicolor.

Authors:  Bhavesh V Mistry; Ricardo Del Sol; Chris Wright; Kim Findlay; Paul Dyson
Journal:  J Bacteriol       Date:  2008-06-13       Impact factor: 3.490

4.  Carboxyethylarginine synthase genes show complex cross-regulation in Streptomyces clavuligerus.

Authors:  Thomas Kwong; Kapil Tahlan; Cecilia L Anders; Susan E Jensen
Journal:  Appl Environ Microbiol       Date:  2012-10-26       Impact factor: 4.792

5.  Cloning and expression of a gene from Streptomyces scabies encoding a putative pathogenicity factor.

Authors:  R A Bukhalid; R Loria
Journal:  J Bacteriol       Date:  1997-12       Impact factor: 3.490

6.  The ATPase ClpX is conditionally involved in the morphological differentiation of Streptomyces lividans.

Authors:  J Viala; P Mazodier
Journal:  Mol Genet Genomics       Date:  2003-01-15       Impact factor: 3.291

7.  Identification by heterologous expression and gene disruption of VisA as L-lysine 2-aminotransferase essential for virginiamycin S biosynthesis in Streptomyces virginiae.

Authors:  Wises Namwat; Hiroshi Kinoshita; Takuya Nihira
Journal:  J Bacteriol       Date:  2002-09       Impact factor: 3.490

8.  In vivo analysis of HPr reveals a fructose-specific phosphotransferase system that confers high-affinity uptake in Streptomyces coelicolor.

Authors:  Harald Nothaft; Stephan Parche; Annette Kamionka; Fritz Titgemeyer
Journal:  J Bacteriol       Date:  2003-02       Impact factor: 3.490

9.  Deletion of a cyclic AMP receptor protein homologue diminishes germination and affects morphological development of Streptomyces coelicolor.

Authors:  A Derouaux; S Halici; H Nothaft; T Neutelings; G Moutzourelis; J Dusart; F Titgemeyer; S Rigali
Journal:  J Bacteriol       Date:  2004-03       Impact factor: 3.490

10.  The phosphotransferase system of Streptomyces coelicolor is biased for N-acetylglucosamine metabolism.

Authors:  Harald Nothaft; Dagmar Dresel; Andreas Willimek; Kerstin Mahr; Michael Niederweis; Fritz Titgemeyer
Journal:  J Bacteriol       Date:  2003-12       Impact factor: 3.490
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