Cloning and sequencing of the beta-glucosidase-encoding gene from Candida molischiana strain 35M5N.

We have isolated a gene (bgln) encoding beta-glucosidase (beta Glu) from a cosmid library of the yeast, Candida molischiana 35M5N. The nucleotide sequence of bgln and its flanking regions was determined. This gene was found to be composed of 2289 bp and 763 amino acid (aa) residues encoding an 83.3-kDa protein. The aa sequence shared eleven putative N-glycosylation sites. Homology comparisons showed that this enzyme can be considered as a new member of the family-3 glycosyl hydrolases. Multiple alignment experiments revealed four conserved regions on aa sequences from beta Glu of this family.