Literature DB >> 748918

Purification of adenine phosphoribosyltransferase by affinity chromatography.

H V Hershey, M W Taylor.   

Abstract

The purine salvage pathway enzyme adenine phosphoribosyltransferase (AMP: pyrophosphate phosphoribosyltransferase EC 2.42.7) has been purified to greater than 85% homogeneity from crude rat liver 100,000 x g supernatant in one step by affinity chromatography. The enzyme binds to an AMP-agarose column and is eluted off the column by 1 mM 5-phosphoribosyl pyrophosphate with a 50 to 80% recovery. Enzyme kinetics indicate that the mechanism of the specific elution is due to competition of the product AMP and substrate 5-phosphoribosyl pyrophosphate for the same site on the enzyme.

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Year:  1978        PMID： 748918     DOI： 10.1080/00327487808061662

Source DB:  PubMed          Journal:  Prep Biochem        ISSN： 0032-7484


  8 in total

1.  APT1, but not APT2, codes for a functional adenine phosphoribosyltransferase in Saccharomyces cerevisiae.

Authors:  J D Alfonzo; T R Crother; M L Guetsova; B Daignan-Fornier; M W Taylor
Journal:  J Bacteriol       Date:  1999-01       Impact factor: 3.490

2.  Identification of DNA sequences required for mouse APRT gene expression.

Authors:  M K Dush; M R Briggs; M E Royce; D A Schaff; S A Khan; J A Tischfield; P J Stambrook
Journal:  Nucleic Acids Res       Date:  1988-09-12       Impact factor: 16.971

3.  Isolation and characterization of the Saccharomyces cerevisiae XPT1 gene encoding xanthine phosphoribosyl transferase.

Authors:  M L Guetsova; T R Crother; M W Taylor; B Daignan-Fornier
Journal:  J Bacteriol       Date:  1999-05       Impact factor: 3.490

4.  Genetic instability at the adenine phosphoribosyltransferase locus in mouse L cells.

Authors:  J A Tischfield; J J Trill; Y I Lee; K Coy; M W Taylor
Journal:  Mol Cell Biol       Date:  1982-03       Impact factor: 4.272

5.  Nucleotide sequence and organization of the mouse adenine phosphoribosyltransferase gene: presence of a coding region common to animal and bacterial phosphoribosyltransferases that has a variable intron/exon arrangement.

Authors:  M K Dush; J M Sikela; S A Khan; J A Tischfield; P J Stambrook
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

6.  Mechanism of adenine toxicity in Escherichia coli.

Authors:  R A Levine; M W Taylor
Journal:  J Bacteriol       Date:  1982-03       Impact factor: 3.490

7.  Metabolism of hypoxanthine in isolated rat hepatocytes.

Authors:  M F Vincent; G Van den Berghe; H G Hers
Journal:  Biochem J       Date:  1984-08-15       Impact factor: 3.857

8.  Model involving gene inactivation in the generation of autosomal recessive mutants in mammalian cells in culture.

Authors:  A E Simon; M W Taylor; W E Bradley; L H Thompson
Journal:  Mol Cell Biol       Date:  1982-09       Impact factor: 4.272
  8 in total

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