Determination of binding constants of Ca2+, Na+, and Cl- ions to liposomal membranes of dipalmitoylphosphatidylcholine at gel phase by particle electrophoresis.

The zeta potentials of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) liposomes were measured at a gel phase as a function of CaCl2 concentration (0-200 mM) in a solution containing different NaCl concentrations (0-200 mM). The data obtained were analyzed with the diffuse double layer theory including the Graham theory. The intrinsic binding constants of ions to DPPC membranes and the distance of the shear plane have been determined independent of both the concentration of CaCl2 and that of NaCl. The values of the constants were 37 M-1 for Ca2+, 0.28 M-1 for Cl, and 0.25 M-1 for Na+; the distance was 0.24 nm under the assumption of binding stoichiometry of Ca2+/DPPC = 1:1.