BACKGROUND: Several enzymatic systems, including glutathione S-transferases, are involved in the metabolism of environmental agents. The absence of glutathione S-transferases mu (GSTM1) and theta (GSTT1) results in decreased detoxification of carcinogens, for example, chemicals in cigarette smoke. These metabolic deficiencies may predispose individuals to the development of smoking-related tumors, such as cancers of the lung, head and neck, and bladder. METHODS: The glutathione S-transferase genotypes of 186 previously untreated patients with squamous cell carcinoma of the head and neck and 42 healthy controls were determined with polymerase chain reaction (PCR) methodologies. Lymphocytes separated from heparinized peripheral blood or whole blood extracts served as sources of genomic DNA. The presence or absence of the gene-specific PCR products revealed the positive or negative genotypes, respectively. RESULTS: The absence of the GSTM1 genotype conferred an odds ratio of 2.37, and the 95% confidence interval (CI) was 1.20 to 4.67. The absence of the GSTT1 gene conferred an odds ratio of 1.47 (CI 0.71 to 3.02). In the population of 42 patients and their matched 42 controls, the absence of the GSTM1 and GSTT1 genotypes conferred odds ratios of 3.10 (CI 1.24 to 7.75) and 2.18 (CI 0.91 to 5.23), respectively. CONCLUSIONS: Despite the small study size, our preliminary data suggest that genetically determined factors of carcinogen metabolism may be associated with increased risk for head and neck cancer.
BACKGROUND: Several enzymatic systems, including glutathione S-transferases, are involved in the metabolism of environmental agents. The absence of glutathione S-transferases mu (GSTM1) and theta (GSTT1) results in decreased detoxification of carcinogens, for example, chemicals in cigarette smoke. These metabolic deficiencies may predispose individuals to the development of smoking-related tumors, such as cancers of the lung, head and neck, and bladder. METHODS: The glutathione S-transferase genotypes of 186 previously untreated patients with squamous cell carcinoma of the head and neck and 42 healthy controls were determined with polymerase chain reaction (PCR) methodologies. Lymphocytes separated from heparinized peripheral blood or whole blood extracts served as sources of genomic DNA. The presence or absence of the gene-specific PCR products revealed the positive or negative genotypes, respectively. RESULTS: The absence of the GSTM1 genotype conferred an odds ratio of 2.37, and the 95% confidence interval (CI) was 1.20 to 4.67. The absence of the GSTT1 gene conferred an odds ratio of 1.47 (CI 0.71 to 3.02). In the population of 42 patients and their matched 42 controls, the absence of the GSTM1 and GSTT1 genotypes conferred odds ratios of 3.10 (CI 1.24 to 7.75) and 2.18 (CI 0.91 to 5.23), respectively. CONCLUSIONS: Despite the small study size, our preliminary data suggest that genetically determined factors of carcinogen metabolism may be associated with increased risk for head and neck cancer.
Authors: Gustavo Jacob Lourenço; Erika Furquim Soledade Neves Silva; José Augusto Rinck-Junior; Carlos Takahiro Chone; Carmen Silvia Passos Lima Journal: Tumour Biol Date: 2011-08-26
Authors: G Cadoni; S Boccia; L Petrelli; P Di Giannantonio; D Arzani; A Giorgio; E De Feo; M Pandolfini; P Gallì; G Paludetti; G Ricciardi Journal: Acta Otorhinolaryngol Ital Date: 2012-02 Impact factor: 2.124
Authors: S Lizard-Nacol; B Coudert; P Colosetti; J M Riedinger; P Fargeot; P Brunet-Lecomte Journal: Breast Cancer Res Date: 1999-09-01 Impact factor: 6.466
Authors: Leonor Varela-Lema; Emanuela Taioli; Alberto Ruano-Ravina; Juan M Barros-Dios; Devasena Anantharaman; Simone Benhamou; Stefania Boccia; Rajani A Bhisey; Gabriella Cadoni; Ettore Capoluongo; Chien-Jen Chen; William Foulkes; Eny Maria Goloni-Bertollo; Ana Hatagima; Richard B Hayes; Takahiko Katoh; Sergio Koifman; Phillip Lazarus; Johannes J Manni; Manoj Mahimkar; Shunji Morita; Jong Park; Kwang-Kyun Park; Erika Cristina Pavarino Bertelli; Enilze Maria de Souza Fonseca Ribeiro; Bidyut Roy; Margaret R Spitz; Richard C Strange; Qingyi Wei; Camille C Ragin Journal: Genet Med Date: 2008-06 Impact factor: 8.822
Authors: Jobaida Akther; Akio Ebihara; Tsutomu Nakagawa; Laila N Islam; Fumiaki Suzuki; Md Ismail Hosen; Mahmud Hossain; A H M Nurun Nabi Journal: Biomed Res Int Date: 2016-05-12 Impact factor: 3.411