G L Naimey1, R B Wuerker. 1. Veterans Affairs Medical Center Long Beach, CA 90822, USA.
Abstract
OBJECTIVE: To evaluate commonly used histologic stains used to detect Pneumocystis, including Diff-Quik (DQ), Grocott's Gomori methenamine silver (GMS), Papanicolaou, and hematoxylin and eosin (H&E). STUDY DESIGN: The study compared the ability of these stains to detect alveolar casts. RESULTS: Although there was a slightly higher average number of casts detected with DQ, the difference between this stain and GMS was not significant. Both Papanicolaou stain and H&E showed significantly lower averages. Some differences in H&E may be related to differences in preparation. CONCLUSION: Similar results were obtained with DQ and GMS, but DQ offered the advantage of ease of preparation, rapidity and cost-effectiveness.
OBJECTIVE: To evaluate commonly used histologic stains used to detect Pneumocystis, including Diff-Quik (DQ), Grocott's Gomori methenamine silver (GMS), Papanicolaou, and hematoxylin and eosin (H&E). STUDY DESIGN: The study compared the ability of these stains to detect alveolar casts. RESULTS: Although there was a slightly higher average number of casts detected with DQ, the difference between this stain and GMS was not significant. Both Papanicolaou stain and H&E showed significantly lower averages. Some differences in H&E may be related to differences in preparation. CONCLUSION: Similar results were obtained with DQ and GMS, but DQ offered the advantage of ease of preparation, rapidity and cost-effectiveness.
Authors: G W Procop; S Haddad; J Quinn; M L Wilson; N G Henshaw; L B Reller; R L Artymyshyn; M T Katanik; M P Weinstein Journal: J Clin Microbiol Date: 2004-07 Impact factor: 5.948