Literature DB >> 7483807

Immunogenicity of influenza A virus N2 neuraminidase produced in insect larvae by baculovirus recombinants.

B E Johansson1, P M Price, E D Kilbourne.   

Abstract

Influenza A virus neuraminidase (NA) from A/Udorn/72 (H3N2) was expressed by recombinant baculovirus-infected insects. The recombinant NA was enzymatically active. Enzyme activity was neutralized by polyclonal antisera raised against virion-extracted NA. NA produced in whole insects by a baculovirus expression system is antigenically indistinguishable from virion NA by polyclonal antisera in functional assays (NI) and in ELISA, and is highly immunogenic without adjuvant. It is equivalent in immunogenicity to NA purified from influenza virus. Our results indicate that baculovirus-produced NA could be used as a source for large quantities of purified N2-NA for vaccine use.

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Year:  1995        PMID： 7483807     DOI： 10.1016/0264-410x(94)00071-t

Source DB:  PubMed          Journal:  Vaccine        ISSN： 0264-410X            Impact factor:   3.641


  9 in total

Review 1.  Influenza vaccines. A reappraisal of their use.

Authors:  A M Palache
Journal:  Drugs       Date:  1997-12       Impact factor: 9.546

2.  Expression, purification and characterization of low-glycosylation influenza neuraminidase in α-1,6-mannosyltransferase defective Pichia pastoris.

Authors:  Yi-Li Yang; Shao-Hong Chang; Xin Gong; Jun Wu; Bo Liu
Journal:  Mol Biol Rep       Date:  2011-05-13       Impact factor: 2.316

3.  Evaluation of antibody response in mice against avian influenza A (H5N1) strain neuraminidase expressed in yeast Pichia pastoris.

Authors:  Murugan Subathra; Ponsekaran Santhakumar; Mangamoori Lakshmi Narasu; Syed Sultan Beevi; Sunil K Lal
Journal:  J Biosci       Date:  2014-06       Impact factor: 1.826

Review 4.  Current research on influenza and other respiratory viruses: II international symposium.

Authors:  F M Munoz; G J Galasso; J M Gwaltney; F G Hayden; B Murphy; R Webster; P Wright; R B Couch
Journal:  Antiviral Res       Date:  2000-05       Impact factor: 5.970

Review 5.  Developments of subunit and VLP vaccines against influenza A virus.

Authors:  Ma-ping Deng; Zhi-hong Hu; Hua-lin Wang; Fei Deng
Journal:  Virol Sin       Date:  2012-06-09       Impact factor: 4.327

6.  Design of the Recombinant Influenza Neuraminidase Antigen Is Crucial for Its Biochemical Properties and Protective Efficacy.

Authors:  Jin Gao; Laura Klenow; Lisa Parsons; Tahir Malik; Je-Nie Phue; Zhizeng Gao; Stephen G Withers; John Cipollo; Robert Daniels; Hongquan Wan
Journal:  J Virol       Date:  2021-10-06       Impact factor: 5.103

7.  Influenza Neuraminidase Subtype N1: Immunobiological Properties and Functional Assays for Specific Antibody Response.

Authors:  Don Changsom; Hatairat Lerdsamran; Witthawat Wiriyarat; Warunya Chakritbudsabong; Bunpote Siridechadilok; Jarunee Prasertsopon; Pirom Noisumdaeng; Wanibtisam Masamae; Pilaipan Puthavathana
Journal:  PLoS One       Date:  2016-04-07       Impact factor: 3.240

Review 8.  Influenza Neuraminidase: A Neglected Protein and Its Potential for a Better Influenza Vaccine.

Authors:  Luca T Giurgea; David M Morens; Jeffery K Taubenberger; Matthew J Memoli
Journal:  Vaccines (Basel)       Date:  2020-07-23

9.  Rotavirus A-specific single-domain antibodies produced in baculovirus-infected insect larvae are protective in vivo.

Authors:  Silvia Gómez-Sebastián; Maria C Nuñez; Lorena Garaicoechea; Carmen Alvarado; Marina Mozgovoj; Rodrigo Lasa; Alan Kahl; Andres Wigdorovitz; Viviana Parreño; José M Escribano
Journal:  BMC Biotechnol       Date:  2012-09-07       Impact factor: 2.563
  9 in total

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