| Literature DB >> 7480201 |
Y Kobayashi1, Y Yamashita, N Fujii, K Takaboshi, T Kawakami, M Kawamura, T Mizukami, H Nakano.
Abstract
A water extract of Coptis chinensis was found to have the ability to stabilize the cleavable complex with mammalian DNA topoisomerase I. As the result of bioassay-guided fractionation, two protoberberine alkaloids, epiberberine and groenlandicine, were identified as active principles with topoisomerase I-mediated DNA cleavage activity in vitro. These two alkaloids did not induce topoisomerase II-mediated DNA cleavage. During further examination of the structurally related protoberberine alkaloids, berberrubine which is produced during the processing of Coptis rhizome as traditional medicine, was identified as a specific inducer of topoisomerase II-mediated DNA cleavage in vitro. These results indicated that protoberberine alkaloids are a chemical family which can induce cleavable complexes with topoisomerases I and II.Entities:
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Year: 1995 PMID： 7480201 DOI： 10.1055/s-2006-958127
Source DB: PubMed Journal: Planta Med ISSN： 0032-0943 Impact factor: 3.352