| Literature DB >> 7480082 |
K Becker1, I Heinroth-Hoffmann, C Giessler, K Pönicke, O E Brodde.
Abstract
The aim of this study was to find out whether, in neonatal rat cardiomyocytes, platelet-activating factor (PAF) can stimulate eicosanoid formation. For this purpose neonatal cardiomyocytes were incubated for 60 min at 37 degrees C in HANKS buffer with PAF (10-1000 nM), and the eicosanoids thromboxane A2 (TXA2) and prostacyclin (PGI2) were assessed in the supernatant as TXB2 and 6-keto-PGF1 alpha, respectively, by an enzyme immunoassay. PAF caused concentration-dependent release of PGI2; TXA2, however, was significantly released only at the highest concentration of PAF (1000 nM). Acetylsalicylic acid (556 microM) and the PAF antagonist WEB 2086 (10 microM) significantly attenuated PAF-induced eicosanoid formation. We conclude that in neonatal rat cardiomyocytes PAF can induce eicosanoid formation and this effect is brought about by activation of a specific PAF receptor.Entities:
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Year: 1995 PMID： 7480082 DOI： 10.1016/0952-3278(95)90116-7
Source DB: PubMed Journal: Prostaglandins Leukot Essent Fatty Acids ISSN： 0952-3278 Impact factor: 4.006