| Literature DB >> 7479734 |
E J Smart1, Y S Ying, C Mineo, R G Anderson.
Abstract
Current methods for purifying caveolae from tissue culture cells take advantage of the Triton X-100 insolubility of this membrane domain. To circumvent the use of detergents, we have developed a method that depends upon the unique buoyant density of caveolae membrane. The caveolae fractions that we obtain are highly enriched in caveolin. As a consequence we are able to identify caveolae-associated proteins that had previously gone undetected. Moreover, resident caveolae proteins that are soluble in Triton X-100 are retained during the isolation.Entities:
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Year: 1995 PMID： 7479734 PMCID： PMC40744 DOI： 10.1073/pnas.92.22.10104
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205