Literature DB >> 7473679

Automated laser fluorescence analysis of randomly amplified polymorphic DNA: a rapid method for investigating nosocomial transmission of Acinetobacter baumannii.

H Grundmann1, C Schneider, H V Tichy, R Simon, I Klare, D Hartung, F D Daschner.   

Abstract

A rapid method for genotyping Acinetobacter baumannii based on PCR-fingerprinting with fluorescent primers was evaluated. Automated laser fluorescence analysis (ALFA) enabled on-line generation of high resolution DNA-fingerprints during polyacrylamide gel electrophoresis of randomly amplified polymorphic DNA (RAPD) products. The results were in concordance with macro-restriction fragment patterns produced by pulse-field gel electrophoresis (PFGE) of ApaI digests of chromosomal DNA. RAPD-ALFA was able to identify homologous strains suggestive of horizontal transmission in < 8 h after colonies were obtained on solid media, whereas PFGE analysis took c. 90 h. Speed and digitised data format renders RAPD-ALFA attractive for routine in-house epidemiological screening of isolates from intensive care and other hospital units.

Entities:  

Mesh：

Substances：

Year:  1995        PMID： 7473679     DOI： 10.1099/00222615-43-6-446

Source DB:  PubMed          Journal:  J Med Microbiol        ISSN： 0022-2615            Impact factor:   2.472


  9 in total

1.  Multicenter evaluation of epidemiological typing of methicillin-resistant Staphylococcus aureus strains by repetitive-element PCR analysis. The European Study Group on Epidemiological Markers of the ESCMID.

Authors:  A Deplano; A Schuermans; J Van Eldere; W Witte; H Meugnier; J Etienne; H Grundmann; D Jonas; G T Noordhoek; J Dijkstra; A van Belkum; W van Leeuwen; P T Tassios; N J Legakis; A van der Zee; A Bergmans; D S Blanc; F C Tenover; B C Cookson; G O'Neil; M J Struelens
Journal:  J Clin Microbiol       Date:  2000-10       Impact factor: 5.948

2.  Comparative evaluation of three different genotyping methods for investigation of nosocomial outbreaks of Legionnaires' disease in hospitals.

Authors:  D Jonas; H G Meyer; P Matthes; D Hartung; B Jahn; F D Daschner; B Jansen
Journal:  J Clin Microbiol       Date:  2000-06       Impact factor: 5.948

3.  Rapid and accurate identification of Staphylococcus species by tRNA intergenic spacer length polymorphism analysis.

Authors:  N Maes; Y De Gheldre; R De Ryck; M Vaneechoutte; H Meugnier; J Etienne; M J Struelens
Journal:  J Clin Microbiol       Date:  1997-10       Impact factor: 5.948

4.  Epidemiology of infections due to multiresistant Enterobacter aerogenes in a university hospital.

Authors:  F Allerberger; T Koeuth; C Lass-FLörl; M P Dierich; C Putensen; E Scmutzhard; I Mohsenipour; H Grundmann; D Hartung; A Bauernfeind; E Eberlein; J R Lupski
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1996-06       Impact factor: 3.267

Review 5.  DNA fingerprinting techniques for microorganisms. A proposal for classification and nomenclature.

Authors:  M Vaneechoutte
Journal:  Mol Biotechnol       Date:  1996-10       Impact factor: 2.695

6.  Acinetobacter species identification by using tRNA spacer fingerprinting.

Authors:  B Ehrenstein; A T Bernards; L Dijkshoorn; P Gerner-Smidt; K J Towner; P J Bouvet; F D Daschner; H Grundmann
Journal:  J Clin Microbiol       Date:  1996-10       Impact factor: 5.948

7.  Multicenter study using standardized protocols and reagents for evaluation of reproducibility of PCR-based fingerprinting of Acinetobacter spp.

Authors:  H J Grundmann; K J Towner; L Dijkshoorn; P Gerner-Smidt; M Maher; H Seifert; M Vaneechoutte
Journal:  J Clin Microbiol       Date:  1997-12       Impact factor: 5.948

8.  Fluorescence-based DNA fingerprinting elucidates nosocomial transmission of phenotypically variable Pseudomonas aeruginosa in intensive care units.

Authors:  H Grundmann; C Schneider; F D Daschner
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1995-12       Impact factor: 3.267

9.  Surveillance of an adult intensive care unit for long-term persistence of a multi-resistant strain of Acinetobacter baumannii.

Authors:  C A Webster; M Crowe; H Humphreys; K J Towner
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1998-03       Impact factor: 3.267
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.