Literature DB >> 7470094

Separation of collagen cyanogen bromide-derived peptides by reversed-phase high-performance liquid chromatography.

M van der Rest, H P Bennett, S Solomon, F H Glorieux.   

Abstract

A new technique for separation of the CNBr cleavage products of collagen is described. It involves the use of a 30 nm pore reversed-phase high-performance liquid chromatography column eluted with a linear gradient of acetonitrile/water containing 0.01 M-heptafluorobutyric acid. The separation of type I, type II and type III CNBr peptides is described. Resolution is particularly good for the low-molecular-weight peptides. The method is fast, quantitative and sensitive, and the complete volatility of the eluent facilitates the recovery of the separated peptides.

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Year:  1980        PMID： 7470094      PMCID： PMC1162205          DOI： 10.1042/bj1910253

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  11 in total

Review 1.  The primary structure of collagen.

Authors:  P P Fietzek; K Kühn
Journal:  Int Rev Connect Tissue Res       Date:  1976

2.  The cyanogen bromide peptides of bovine soluble and insoluble collagens. I. Characterization of peptides from soluble type I collagen by sodium dodecylsulphate polyacrylamide gel electrophoresis.

Authors:  P G Scott; A Veis
Journal:  Connect Tissue Res       Date:  1976       Impact factor: 3.417

3.  Isolation and characterization of the cyanogen bromide peptides from the alpha-1 chain of rat skin collagen.

Authors:  W T Butler; K A Piez; P Bornstein
Journal:  Biochemistry       Date:  1967-12       Impact factor: 3.162

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Equilibrium and kinetic studies of the helix-coil transition in alpha 1-CB2, a small peptide from collagen.

Authors:  K A Piez; M R Sherman
Journal:  Biochemistry       Date:  1970-10-13       Impact factor: 3.162

6.  Analysis of collagen cyanogen bromide peptides using electrophoresis in continuous concave gradient polyacrylamide gels.

Authors:  W G Cole; D A Bean
Journal:  Anal Biochem       Date:  1979-01-01       Impact factor: 3.365

7.  The nature of the intramolecular cross-links in collagen. The separation and characterization of peptides from the cross-link region of rat skin collagen.

Authors:  P Bornstein; K A Piez
Journal:  Biochemistry       Date:  1966-11       Impact factor: 3.162

8.  Characterisation of the major CNBr-Derived peptides of porcine type II collagen.

Authors:  D R Eyre; H Muir
Journal:  Connect Tissue Res       Date:  1975       Impact factor: 3.417

9.  Human collagen 'fingerprints' produced by clostridopeptidase A digestion and high-pressure liquid chromatography.

Authors:  M van Der Rest; W G Cole; F H Glorieux
Journal:  Biochem J       Date:  1977-03-01       Impact factor: 3.857

10.  Ordering of cyanogen bromide peptides of type III collagen based on their homology to type I collagen: preservation of sites for crosslink formation during evolution.

Authors:  P P Fietzek; H Allmann; J Rauterberg; E Wachter
Journal:  Proc Natl Acad Sci U S A       Date:  1977-01       Impact factor: 11.205
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  2 in total

1.  The behaviour of peptides on reverse-phase supports during high-pressure liquid chromatography.

Authors:  K J Wilson; A Honegger; R P Stötzel; G J Hughes
Journal:  Biochem J       Date:  1981-10-01       Impact factor: 3.857

2.  Comparison of buffers and detection systems for high-pressure liquid chromatography of peptide mixtures.

Authors:  K J Wilson; A Honegger; G J Hughes
Journal:  Biochem J       Date:  1981-10-01       Impact factor: 3.857
  2 in total

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