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Literature DB >> 7470062

Alkaline phosphatase activity does not mediate phosphate transport in the renal-cortical brush-border membrane.

Abstract

We studied (1) the effect of primary modulators of phosphate transport, namely the hypophosphataemic mouse mutant (Hyp) and low-phosphorus diet, on alkaline phosphatase activity in mouse renal-cortex brush-border membrane vesicles and (2) the effect of several primary inhibitors of alkaline phosphatase on phosphate transport. Brush-border membrane vesicles from Hyp-mouse kidney had 50% loss of Na+-dependent phosphate transport, but only 18% decrease in alkaline phosphatase activity. The low-phosphorus diet effectively stimulated Na+/phosphate co-transport in brush-border membrane vesicles (+ 118%), but increased alkaline phosphatase activity only slightly (+13%). Levamisole (0.1 mM) and EDTA (1.0 mM) inhibited brush-border membrane-vesicle alkaline phosphatase activity of 82% and 93% respectively, but had no significant effect on Na+/phosphate co-transport. We conclude that alkaline phosphatase does not play a direct role in phosphate transport across the brush-border membrane of mouse kidney.

Entities: Chemical Species

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Year: 1980 PMID： 7470062 PMCID： PMC1162116 DOI： 10.1042/bj1900473

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

17 in total

1. Renal handling of phosphate in vivo and in vitro by the X-linked hypophosphatemic male mouse: evidence for a defect in the brush border membrane.

Authors: H S Tenenhouse; C R Scriver; R R McInnes; F H Glorieux
Journal: Kidney Int Date: 1978-09 Impact factor: 10.612

Review 2. Microvilli: their ultrastructure, enzymology and molecular organization.

Authors: A J Kenny; A G Booth
Journal: Essays Biochem Date: 1978 Impact factor: 8.000

3. Kinetics and inhibition of alkaline phosphatases from canine tissues.

Authors: H Van Belle
Journal: Biochim Biophys Acta Date: 1972-11-10

4. Phosphate absorption and alkaline phosphatase activity in the small intestine of the adult mouse and of the chick embryo and hatched chick.

Authors: F Moog; H S Glazier
Journal: Comp Biochem Physiol A Comp Physiol Date: 1972-06-01

5. A rapid method for the preparation of microvilli from rabbit kidney.

Authors: A G Booth; A J Kenny
Journal: Biochem J Date: 1974-09 Impact factor: 3.857

6. Studies on the enzymology of purified preparations of brush border from rabbit kidney.

Authors: S G George; J Kenny
Journal: Biochem J Date: 1973-05 Impact factor: 3.857

7. The effect of several diphosphonates on acid phosphohydrolases and other lysosomal enzymes.

Authors: R Felix; R Graham; G Russell; H Fleisch
Journal: Biochim Biophys Acta Date: 1976-04-08

8. Phenylalanine uptake in isolated renal brush border vesicles.

Authors: J Evers; H Murer; R Kinne
Journal: Biochim Biophys Acta Date: 1976-04-05

9. Hypophosphatemia: mouse model for human familial hypophosphatemic (vitamin D-resistant) rickets.

Authors: E M Eicher; J L Southard; C R Scriver; F H Glorieux
Journal: Proc Natl Acad Sci U S A Date: 1976-12 Impact factor: 11.205

10. The defect in transcellular transport of phosphate in the nephron is located in brush-border membranes in X-linked hypophosphatemia (Hyp mouse model).

Authors: H S Tenenhouse; C R Scriver
Journal: Can J Biochem Date: 1978-06

8 in total

1. Relation between intestinal alkaline phosphatase activity and brush border membrane transport of inorganic phosphate, D-glucose, and D-glucose-6-phosphate.

Authors: C Roubaty; P Portmann
Journal: Pflugers Arch Date: 1988-10 Impact factor: 3.657

2. Membrane transport in the proximal tubule and thick ascending limb of Henle's loop: mechanisms and their alterations.

Authors: H Murer; R Greger
Journal: Klin Wochenschr Date: 1982-09-15

3. Characterization of phosphate binding by alkaline phosphatase in rat kidney brush border membrane.

Authors: R Beliveau; M G Brunette; J Strevey
Journal: Pflugers Arch Date: 1983-08 Impact factor: 3.657

4. The response of alkaline phosphatase to osmoregulatory changes in the trout, Salmo gairdneri.

Authors: K W Gasser; L B Kirschner
Journal: J Comp Physiol B Date: 1987 Impact factor: 2.200

5. Hydrolysis of nicotinamide-adenine dinucleotide by purified renal brush-border membranes. Mechanism of NAD+ inhibition of brush-border membrane phosphate-transport activity.

Authors: H S Tenenhouse; Y L Chu
Journal: Biochem J Date: 1982-06-15 Impact factor: 3.857