| Literature DB >> 7470060 |
Abstract
An enzyme catalysing the synthesis of sym-homospermidine from putrescine and NAD+ with concomitant liberation of NH3 was purified 100-fold from Lathyrus sativus (grass pea) seedlings by affinity chromatography on Blue Sepharose. This thiol enzyme had an apparent mol.wt. of 75000 and exhibited Michelis-Menten kinetics with Km 3.0mM for putrescine. The same enzyme activity could also be demonstrated in the crude extracts of sandal (Santalum album) leaves, but with a specific activity 15-fold greater than that in L. sativus seedlings.Entities:
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Year: 1980 PMID: 7470060 PMCID: PMC1162113 DOI: 10.1042/bj1900461
Source DB: PubMed Journal: Biochem J ISSN: 0264-6021 Impact factor: 3.857