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Literature DB >> 7465425

Purification and partial characterization of a DNA polymerase alpha species from calf thymus.

Abstract

We have purified a DNA polymerase alpha species from calf thymus to near homogeneity. The enzyme sediments at 5.7 S and contains two polypeptides of 123000 and 134000 daltons in about equimolar ratio. The enzyme is inhibited by aphidicolin and N-ethylmaleimide, and retains its activity in buffers containing moderate salt conditions. Activated DNA is a better substrate than poly-(dA) . (dT) 10.

Entities: Chemical Species

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Year: 1980 PMID： 7465425 PMCID： PMC324335 DOI： 10.1093/nar/8.23.5703

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

18 in total

1. Evidence that DNA polymerase-alpha of calf thymus contains a subunit of molecular weight 155000.

Authors: A M Holmes; I P Hesslewood; I R Johnston
Journal: Eur J Biochem Date: 1976-02-16

2. Studies on the purification and properties of a 6.8-S DNA polymerase activity found in calf-thymus DNA polymerase-alpha fraction.

Authors: I P Hesslewood; A M Holmes; W F Wakeling; I R Johnston
Journal: Eur J Biochem Date: 1978-03

3. DNA polymerase-alpha. Purification and structural characterization of the near homogeneous enzyme from human KB cells.

Authors: P A Fisher; D Korn
Journal: J Biol Chem Date: 1977-09-25 Impact factor: 5.157

4. Affinity chromatography of phosphofructokinase using Cibacron blue F3G-A.

Authors: H J Böhme; G Kopperschläger; J Schulz; E Hofmann
Journal: J Chromatogr Date: 1972-06-28

5. Nucleotide polymerizing enzymes from calf thymus gland.

Authors: F J Bollum; L M Chang; C M Tsiapalis; J W Dorson
Journal: Methods Enzymol Date: 1974 Impact factor: 1.600

6. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.

Authors: R R Burgess
Journal: J Biol Chem Date: 1969-11-25 Impact factor: 5.157

9. Exonucleolytic proofreading increases the accuracy of DNA synthesis by human lymphocyte DNA polymerase alpha-DNA primase.

Authors: G Bialek; H P Nasheuer; H Goetz; F Grosse
Journal: EMBO J Date: 1989-06 Impact factor: 11.598

10. A mammalian DNA polymerase alpha holoenzyme functioning on defined in vivo-like templates.

Authors: U Hübscher; P Gerschwiler; G K McMaster
Journal: EMBO J Date: 1982 Impact factor: 11.598

10 in total

Purification and partial characterization of a DNA polymerase alpha species from calf thymus.

1. Evidence that DNA polymerase-alpha of calf thymus contains a subunit of molecular weight 155000.

2. Studies on the purification and properties of a 6.8-S DNA polymerase activity found in calf-thymus DNA polymerase-alpha fraction.

3. DNA polymerase-alpha. Purification and structural characterization of the near homogeneous enzyme from human KB cells.

4. Affinity chromatography of phosphofructokinase using Cibacron blue F3G-A.

5. Nucleotide polymerizing enzymes from calf thymus gland.

6. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.

7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

8. [Purification and properties of phenylalanyl-tRNA synthetase from baker's yeast].

9. A rapid technique to separate DNA polymerase-alpha and -beta activity from a cytosol extract.

10. Characterization of macromolecules by constant velocity sedimentation.

1. Fidelity of human immunodeficiency virus type I reverse transcriptase in copying natural DNA.

Review 2. DNA polymerases in prokaryotes and eukaryotes: mode of action and biological implications.

3. Isolation of the catalytic core of DNA polymerase alpha from rabbit bone marrow.

4. Subspecies of DNA polymerase alpha from calf thymus with different fidelity in copying synthetic template-primers.

5. The elongation of mismatched primers by DNA polymerase alpha from calf thymus.

6. Cell-cycle dependence and properties of the HeLa cell DNA polymerase system.

7. Isolation of an intact DNA polymerase-primase from embryos of Drosophila melanogaster.

8. The mammalian primase is part of a high molecular weight DNA polymerase alpha polypeptide.

9. Exonucleolytic proofreading increases the accuracy of DNA synthesis by human lymphocyte DNA polymerase alpha-DNA primase.

10. A mammalian DNA polymerase alpha holoenzyme functioning on defined in vivo-like templates.