Literature DB >> 7464979

Stability of the glutamate content of synaptosomes during their preparation.

J W Geddes, J D Newstead, J D Wood.   

Abstract

The glutamate content of synaptosomal-enriched fractions is stable during the preparation and isolation of the organelles provided that the temperature of the preparation is maintained at 0--2 degrees C and that inorganic ions are not added to the homogenizing and centrifugation media. Consequently, drug-induced in vivo changes in the glutamate content of nerve endings should be reflected by the corresponding changes in glutamate levels of synaptosomes prepared from the brain tissue.

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Year:  1980        PMID: 7464979     DOI: 10.1007/bf00966168

Source DB:  PubMed          Journal:  Neurochem Res        ISSN: 0364-3190            Impact factor:   3.996


  9 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Stability of synaptosomal GABA levels and their use in determining the in vivo effects of drugs: convulsant agents.

Authors:  J D Wood; M P Russell; E Kurylo; J D Newstead
Journal:  J Neurochem       Date:  1979-07       Impact factor: 5.372

3.  Fluorometric determination of aspartate, glutamate, and gamma-aminobutyrate in nerve tissue using enzymic methods.

Authors:  L T Graham; M H Aprison
Journal:  Anal Biochem       Date:  1966-06       Impact factor: 3.365

4.  Determination of protein: a modification of the Lowry method that gives a linear photometric response.

Authors:  E F Hartree
Journal:  Anal Biochem       Date:  1972-08       Impact factor: 3.365

5.  GABA and glutamate uptake by subcellular fractions enriched in synaptosomes: critical evaluation of some methodological aspects.

Authors:  G Levi; M Raiteri
Journal:  Brain Res       Date:  1973-07-16       Impact factor: 3.252

6.  Metabolism of beds of mammalian cortical synaptosomes: response to depolarizing influences.

Authors:  J S De Belleroche; H F Bradford
Journal:  J Neurochem       Date:  1972-03       Impact factor: 5.372

7.  Glutamine uptake and metabolism by the isolated toad brain: evidence pertaining to its proposed role as a transmitter precursor.

Authors:  R P Shank; M H Aprison
Journal:  J Neurochem       Date:  1977-06       Impact factor: 5.372

8.  The distribution of free amino acids in subcellular fractions of guinea-pig brain.

Authors:  J L Mangan; V P Whittaker
Journal:  Biochem J       Date:  1966-01       Impact factor: 3.857

9.  Metabolic inhibitors and subcellular distribution of GABA.

Authors:  S Sarhan; N Seiler
Journal:  J Neurosci Res       Date:  1979       Impact factor: 4.164

  9 in total

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