| Literature DB >> 7462655 |
G S Eisenbarth, R B Rankin, B F Haynes, A S Fauci.
Abstract
We have developed a visual microtiter assay to detect solubilized cell-surface antigens which react with monoclonal antibodies. The assay depends on the ability of adsorbed monoclonal antibody to bind target cells to microtiter V wells, and the inhibition of binding by antigen. We have used this assay to follow a 600-fold purification of the human lymphocyte differentiation antigen 3A1 extracted from HSB-2 cells. Antigen 3A1 is a glycoprotein with a molecular weight of approximately 40,000 daltons.Entities:
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Year: 1980 PMID： 7462655 DOI： 10.1016/0022-1759(80)90239-2
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303