Literature DB >> 7462647

The isolation and purification of human peripheral blood monocytes in cell suspension.

A J Treves, D Yagoda, A Haimovitz, N Ramu, D Rachmilewitz, Z Fuks.   

Abstract

A simple method for the isolation of human peripheral blood monocytes in cell suspension is described. The method is based on the adherence and detachment characteristics of monocytes during their in vitro cultivation and does not require any gradient centrifugation or modulating substances. The mean percentage of monocytes recovered from different donors was 81.9% (60-100%) of the original phagocytic cells found in the peripheral blood of the tested donors and the cell suspensions obtained consisted of more than 95% of monocytes. The monocytes isolated in suspensions show high activity in various monocyte functions tested.

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Year:  1980        PMID： 7462647     DOI： 10.1016/0022-1759(80)90295-1

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  9 in total

1.  Still's disease associated with adenovirus infection and defect in adenovirus directed natural killing.

Authors:  A S Luder; V Naphtali; E Ben Porat; N Lahat
Journal:  Ann Rheum Dis       Date:  1989-09       Impact factor: 19.103

2.  Quantitative assessment of Fc receptor expression and function during in vitro differentiation of human monocytes to macrophages.

Authors:  T W Jungi; S Hafner
Journal:  Immunology       Date:  1986-05       Impact factor: 7.397

3.  Hydroxyl radical scavengers inhibit human lectin-dependent cellular cytotoxicity.

Authors:  M Melinn; H McLaughlin
Journal:  Immunology       Date:  1986-06       Impact factor: 7.397

4.  Regulation of immunoglobulin synthesis by monocytes in multiple myeloma and benign monoclonal gammopathy.

Authors:  A Takeshita
Journal:  Clin Exp Immunol       Date:  1986-12       Impact factor: 4.330

5.  Prostanoid synthesis by cultured intestinal epithelial and mononuclear cells in inflammatory bowel disease.

Authors:  A Zifroni; A J Treves; D B Sachar; D Rachmilewitz
Journal:  Gut       Date:  1983-07       Impact factor: 23.059

6.  Increased apoptosis of peripheral blood mononuclear cells in patients with perennial allergic asthma/rhinitis: relation to serum markers of apoptosis.

Authors:  Janina Grzegorczyk; Marek L Kowalski; Anna Pilat; Jolanta Iwaszkiewicz
Journal:  Mediators Inflamm       Date:  2002-08       Impact factor: 4.711

7.  Anti- schistosomular activity of human monocytes/macrophages in response to interleukin-3 and granulocyte-macrophage colonystimulating factor stimulation.

Authors:  D Gold; L Nissimov; J Lengy; Y Keisari
Journal:  Mediators Inflamm       Date:  1994       Impact factor: 4.711

8.  Targeting In-Stent-Stenosis with RGD- and CXCL1-Coated Mini-Stents in Mice.

Authors:  Sakine Simsekyilmaz; Elisa A Liehn; Stefan Weinandy; Fabian Schreiber; Remco T A Megens; Wendy Theelen; Ralf Smeets; Stefan Jockenhövel; Thomas Gries; Martin Möller; Doris Klee; Christian Weber; Alma Zernecke
Journal:  PLoS One       Date:  2016-05-18       Impact factor: 3.240

9.  Metformin-induced suppression of IFN-α via mTORC1 signalling following seasonal vaccination is associated with impaired antibody responses in type 2 diabetes.

Authors:  Wipawee Saenwongsa; Arnone Nithichanon; Malinee Chittaganpitch; Kampaew Buayai; Chidchamai Kewcharoenwong; Boonyarat Thumrongwilainet; Patcharavadee Butta; Tanapat Palaga; Yoshimasa Takahashi; Manabu Ato; Ganjana Lertmemongkolchai
Journal:  Sci Rep       Date:  2020-02-24       Impact factor: 4.379
  9 in total

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