| Literature DB >> 7462375 |
Abstract
A high-performance liquid chromatographic system was developed with baseline separation of coenzyme A (CoASH) from dephospho-coenzyme A and acetyl-coenzyme A using isocratic elution. The chromatographic separation was achieved in a reversed-phase system with a high concentration (220 mM) of potassium phosphate buffer at pH 4.0 and appropriate amounts of methanol (ca. 12%). The eluate was monitored with a UV detector at 254 nm with the limit of detection at ca. 5 pmoles. The system could be used without modification for the estimation of the content of CoASH in biological extracts, e.g. mitochondria.Entities:
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Year: 1980 PMID： 7462375 DOI： 10.1016/s0021-9673(00)91829-6
Source DB: PubMed Journal: J Chromatogr