Literature DB >> 7461733

Fc gamma-receptor activity of isolated human placental syncytiotrophoblast plasma membrane.

P J Brown, P M Johnson.   

Abstract

Fc gamma-receptor activity of isolated human placental syncytiotrophoblast microvillous plasma membrane (StMPM) vesicle preparations has been determined in an immunoradiometric assay using Sepharose-immobilized protein A to separate free 125I-labelled human IgG from membrane-bound 125I-IgG. This receptor assay has been optimalized in terms of buffer pH and molarity, and used to demonstrate that prior 60 min washing of isolated membranes in 3 M KCl to remove extrinsic membrane-bound protein substantially increases the membrane-binding capacity for IgG. Inhibition studies have determined the syncytiotrophoblast Fc gamma-receptor equilibrium constant for association (Ka) as 4.0 x 10(7) M-1 at 37 degrees and the number of available Fc gamma-receptor sites as 1.5 x 10(14) per mg membrane protein.

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Year:  1981        PMID： 7461733      PMCID： PMC1458069     

Source DB:  PubMed          Journal:  Immunology        ISSN： 0019-2805            Impact factor:   7.397


  12 in total

1.  Preparation of iodine-131 labelled human growth hormone of high specific activity.

Authors:  W M HUNTER; F C GREENWOOD
Journal:  Nature       Date:  1962-05-05       Impact factor: 49.962

2.  Structure and function of immunoglobulin domains. V. Binding, University of immunoglobulin G and fragments to placental membrane preparations.

Authors:  T McNabb; T Y Koh; K J Dorrington; R H Painter
Journal:  J Immunol       Date:  1976-09       Impact factor: 5.422

3.  Multiple FC receptors in the human placenta.

Authors:  R Matre; P M Johnson
Journal:  Acta Pathol Microbiol Scand C       Date:  1977-08

4.  Preparation of human placental villous surface membrane.

Authors:  N C Smith; M G Brush; S Luckett
Journal:  Nature       Date:  1974-11-22       Impact factor: 49.962

5.  Characterisation of the soluble fraction of human syncytiotrophoblast microvillous plasma membrane-associated proteins.

Authors:  A O Ogbimi; P M Johnson; P J Brown; H Fox
Journal:  J Reprod Immunol       Date:  1979-07       Impact factor: 4.054

6.  The Scatchard plot in immunometric assay.

Authors:  W H Walker
Journal:  Clin Chem       Date:  1977-03       Impact factor: 8.327

7.  Immunological studies of human placentae: subclass and fragment specificity of binding of aggregated IgG by placental endothelial cells.

Authors:  P M Johnson; W P Faulk; A C Wang
Journal:  Immunology       Date:  1976-10       Impact factor: 7.397

8.  The Fc gamma receptor on human placental plasma membrane. I. Studies on the binding of homologous and heterologous immunoglobulin G1.

Authors:  J A van der Meulen; T C McNabb; N Haeffner-Cavaillon; M Klein; K J Dorrington
Journal:  J Immunol       Date:  1980-02       Impact factor: 5.422

9.  Crystallization, crystal structure analysis and atomic model of the complex formed by a human Fc fragment and fragment B of protein A from Staphylococcus aureus.

Authors:  J Deisenhofer; T A Jones; R Huber; J Sjödahl; J Sjöquist
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1978-08

10.  Properties of receptors for IgG on human placental cell membranes.

Authors:  A H Balfour; E A Jones
Journal:  Int Arch Allergy Appl Immunol       Date:  1978
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  3 in total

1.  Expression of placental alkaline phosphatase does not correlate with IgG binding, internalization and transcytosis.

Authors:  I Stefaner; A Stefanescu; W Hunziker; R Fuchs
Journal:  Biochem J       Date:  1997-10-15       Impact factor: 3.857

2.  Rabbit peroxidase-antiperoxidase complex (PAP) as a model for the uptake of immunoglobulin G by the human placenta.

Authors:  G D Griffiths; D Kershaw; A G Booth
Journal:  Histochem J       Date:  1985-08

3.  Isolation and characterization of human fetal macrophages from placenta.

Authors:  L N Sutton; D Y Mason; C W Redman
Journal:  Clin Exp Immunol       Date:  1989-12       Impact factor: 4.330
  3 in total

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