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Literature DB >> 745595

Electron microscopic analysis of in vitro transcriptional complexes: mapping of promoters of the coliphage T5 genome.

Abstract

Transcriptional complexes formed in vitro with coliphage T5 DNA as template were analyzed by electron microscopy and the number and location of starting sites utilized by E. coli RNA polymerase were determined. Of the 40 promoters characterized in this way, 6 map in the two terminal "pre-early" regions, 29 in the "early" and 5 in the "late" region. The direction of transcription within the different regions determined in this study agrees with earlier findings derived from RNA synthesized in vivo.

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Year: 1978 PMID： 745595 DOI： 10.1007/bf00285916

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

18 in total

Review 1. The T-odd bacteriophages.

Authors: D J McCorquodale
Journal: CRC Crit Rev Microbiol Date: 1975-12

2. A new bacteriophage T5 particle with a DNA lacking the terminal repetition at the "right-hand" end.

Authors: K Saigo
Journal: J Mol Biol Date: 1976-11-05 Impact factor: 5.469

3. Denaturation mapping and chromosome structure in bacteriophage T5.

Authors: K Saigo
Journal: Virology Date: 1975-11 Impact factor: 3.616

4. An electron microscopic method for studying nucleic acid-protein complexes. Visualization of RNA polymerase bound to the DNA of bacteriophages T7 and T3.

Authors: T Koller; J M Sogo; H Bujard
Journal: Biopolymers Date: 1974-05 Impact factor: 2.505

5. Structure and function of the genome of coliphage T5. 1. The physical structure of the chromosome of T5 + .

Authors: H Bujard; H E Hendrickson
Journal: Eur J Biochem Date: 1973-03-15

6. Length measurements of RNA synthesized in vitro by Escherichia coli RNA polymerase.

Authors: H Delius; H Westphal; N Axelrod
Journal: J Mol Biol Date: 1973-03-15 Impact factor: 5.469

7. Structure and function of the genome of coliphage T5. 2. Regions of transcription of the chromosome.

Authors: H E Hendrickson; H Bujard
Journal: Eur J Biochem Date: 1973-03-15

8. T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA.

Authors: B M Alberts; L Frey
Journal: Nature Date: 1970-09-26 Impact factor: 49.962

9. Synthesis of bacteriophage T5 specific RNA in vitro.

Authors: J P Pispa; J M Buchanan
Journal: Biochim Biophys Acta Date: 1971-09-30

10. T7 early RNAs are generated by site-specific cleavages.

Authors: J J Dunn; F W Studier
Journal: Proc Natl Acad Sci U S A Date: 1973-05 Impact factor: 11.205

19 in total

1. Electron microscopic analysis of transcription: mapping of initiation sites and direction of transcription.

Authors: C Brack
Journal: Proc Natl Acad Sci U S A Date: 1979-07 Impact factor: 11.205

2. Cloning, sequencing, and recombinational analysis with bacteriophage BF23 of the bacteriophage T5 oad gene encoding the receptor-binding protein.

Authors: V Krauel; K J Heller
Journal: J Bacteriol Date: 1991-02 Impact factor: 3.490

3. Localization of the dihydrofolate reductase gene on the physical map of bacteriophage T5.

Authors: G Peter; U J Hänggi; H G Zachau
Journal: Mol Gen Genet Date: 1979-10-01

4. Promoters recognized by Escherichia coli RNA polymerase selected by function: highly efficient promoters from bacteriophage T5.

Authors: R Gentz; H Bujard
Journal: J Bacteriol Date: 1985-10 Impact factor: 3.490