Literature DB >> 7442665

Physicochemical measurement of the base composition of mRNA-related sequences of the human alpha and beta globin genes.

D Tuan, B G Forget.   

Abstract

Hybrids formed between human alpha and beta globin cDNA and total human cellular DNA have been studied by thermal denaturation and cesium chloride density gradient centrifugation. From these studies, the weight average G + C content of human alpha globin cDNA has been determined to be 62% +/- 2% and that of human beta globin cDNA 51% +/- 2%. These values correlate well with the results of G + C content of the human alpha and beta globin cDNAs as determined by direct nucleotide sequence analysis of the cDNAs. Thermal denaturation and cesium chloride density gradient centrifugation of DNA-cDNA hybrids can therefore provide accurate information on the base composition of mRNA related sequences of any single copy gene for which a relatively pure cDNA can be obtained, without the necessity for direct nucleotide sequence analysis.

Entities:  

Mesh：

Substances：

Year:  1980        PMID： 7442665     DOI： 10.1007/bf00775413

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  16 in total

1.  Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

Authors:  T Maniatis; A Jeffrey; H van deSande
Journal:  Biochemistry       Date:  1975-08-26       Impact factor: 3.162

2.  Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.

Authors:  C L SCHILDKRAUT; J MARMUR; P DOTY
Journal:  J Mol Biol       Date:  1962-06       Impact factor: 5.469

3.  Nucleotide sequence of the coding portion of human alpha globin messenger RNA.

Authors:  J T Wilson; L B Wilson; V B Reddy; C Cavallesco; P K Ghosh; J K deRiel; B G Forget; S M Weissman
Journal:  J Biol Chem       Date:  1980-04-10       Impact factor: 5.157

4.  Nucleotide sequence of 3' untranslated portion of human alpha globin mRNA.

Authors:  J T Wilson; J K deRiel; B G Forget; C A Marotta; S M Weissman
Journal:  Nucleic Acids Res       Date:  1977-07       Impact factor: 16.971

5.  The sequence of the chromosomal mouse beta-globin major gene: homologies in capping, splicing and poly(A) sites.

Authors:  D A Konkel; S M Tilghman; P Leder
Journal:  Cell       Date:  1978-12       Impact factor: 41.582

6.  The complete sequence of a chromosomal mouse alpha--globin gene reveals elements conserved throughout vertebrate evolution.

Authors:  Y Nishioka; P Leder
Journal:  Cell       Date:  1979-11       Impact factor: 41.582

7.  The primary structure of rabbit beta-globin mRNA as determined from cloned DNA.

Authors:  A Efstratiadis; F C Kafatos; T Maniatis
Journal:  Cell       Date:  1977-04       Impact factor: 41.582

8.  Hemoglobin switching in sheep and goats. Preparation and characterization of complementary DNAs specific for the alpha-, beta-, and gamma-globin messenger RNAs of sheep.

Authors:  E J Benz; C E Geist; A W Steggles; J E Barker; A W Nienhuis
Journal:  J Biol Chem       Date:  1977-03-25       Impact factor: 5.157

9.  The primary sequence of rabbit alpha-globin mRNA.

Authors:  H C Heindell; A Liu; G V Paddock; G M Studnicka; W A Salser
Journal:  Cell       Date:  1978-09       Impact factor: 41.582

10.  Human beta-globin messenger RNA. III. Nucleotide sequences derived from complementary DNA.

Authors:  C A Marotta; J T Wilson; B G Forget; S M Weissman
Journal:  J Biol Chem       Date:  1977-07-25       Impact factor: 5.157
View more
  1 in total

1.  Resistance of mRNAs with AUG-proximal nonsense mutations to nonsense-mediated decay reflects variables of mRNA structure and translational activity.

Authors:  Francisco J C Pereira; Alexandre Teixeira; Jian Kong; Cristina Barbosa; Ana Luísa Silva; Ana Marques-Ramos; Stephen A Liebhaber; Luísa Romão
Journal:  Nucleic Acids Res       Date:  2015-06-11       Impact factor: 16.971
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.