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Literature DB >> 7442656

Photokinetic microanalysis of NADP+, using bacterial luciferase.

Abstract

Bioluminescence photokinetic assay of NADP+ is described, using the glucose-6-phosphate dehydrogenase reaction for conversion to its reduced form and subsequent measurement of this with luciferase extracts of Vibria fisherii. the analyses were applied to the determination of the activity of minute amounts of glutathione reductase using NADP+ as measurable product and for nucleotide assay in cell samples of 0.5--10 microgram dry weight. The sensitivity was sufficient for determining 0.5 picomoles NADP+. Previously, FMN, NADH, NAD+ and NADH have been analysed with the bacterial luciferase system. Its applicability has not been extended by the assay of NADP+.

Entities: Chemical Gene

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Year: 1980 PMID： 7442656 DOI： 10.1007/bf00421291

Source DB: PubMed Journal: Mol Cell Biochem ISSN： 0300-8177 Impact factor: 3.396

11 in total

4. Microassay with the NADH-induced light reaction, technique improved by means of purified enzymes from Achromobacter fischeri.

Authors: S E Brolin; S Hjertén
Journal: Mol Cell Biochem Date: 1977-09-09 Impact factor: 3.396

5. Simplified luciferase assay of NAD+ applied to microsamples from liver, kidney and pancreatic islets.

Authors: A Agren; S E Brolin; S Hjertén
Journal: Biochim Biophys Acta Date: 1977-11-07

6. Determination of subpicomole levels of NADH and FMN using bacterial luciferase and the liquid scintillation spectrometer.

Authors: P E Stanley
Journal: Anal Biochem Date: 1971-02 Impact factor: 3.365

1. Single-step bioluminescence analyses of enzymes, using Cibacrone Blue chromatography for removal of interfering dehydrogenases.

Authors: S E Brolin
Journal: Mol Cell Biochem Date: 1983 Impact factor: 3.396

1 in total

Photokinetic microanalysis of NADP+, using bacterial luciferase.

1. Substrate analyses in single cells. I. Determination of ATP.

2. The quantitative histochemistry of the brain; histological sampling.

Review 3. The contribution of microchemical methods of histochemistry to the biological sciences.

4. Microassay with the NADH-induced light reaction, technique improved by means of purified enzymes from Achromobacter fischeri.

5. Simplified luciferase assay of NAD+ applied to microsamples from liver, kidney and pancreatic islets.

6. Determination of subpicomole levels of NADH and FMN using bacterial luciferase and the liquid scintillation spectrometer.

7. Photokinetic micro assay based on dehydrogenase reactions and bacterial luciferase.

8. Photokinetic assay of NADH and NADPH in microdissected tissue samples.

9. Glucose-6-phosphate dehydrogenase, the pentose phosphate cycle, and its place in carbohydrate metabolism.

10. Biochemical micro analysis based upon chemiluminescence.

1. Single-step bioluminescence analyses of enzymes, using Cibacrone Blue chromatography for removal of interfering dehydrogenases.