Literature DB >> 7440247

Criteria of reliability for light microscopic immunocytochemical staining.

P Petrusz, P Ordronneau, J C Finley.   

Abstract

The following criteria of reliability are defined and discussed for immunocytochemical staining at the light microscopical level: efficiency, accuracy, precision, sensitivity, and specificity. Whenever practical, tests are suggested for obtaining information on the extent to which these criteria are fulfilled in a given system, and procedures are outlined for improving immunocytochemical staining in terms of these criteria. It is suggested that consideration of reliability criteria will help investigators in their choice of methodology, design of experimental strategy, and valid interpretation of the results.

Mesh：

Year:  1980        PMID： 7440247     DOI： 10.1007/bf01006954

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  29 in total

1.  Fluorescent antibody studies with agents of varicella and herpes zoster propagated in vitro.

Authors:  T H WELLER; A H COONS
Journal:  Proc Soc Exp Biol Med       Date:  1954 Aug-Sep

2.  Enzyme-labeled antibodies: preparation and application for the localization of antigens.

Authors:  P K Nakane; G B Pierce
Journal:  J Histochem Cytochem       Date:  1966-12       Impact factor: 2.479

Review 3.  A critical review of immunocytochemical methods for light microscopy.

Authors:  F Vandesande
Journal:  J Neurosci Methods       Date:  1979-03       Impact factor: 2.390

4.  Protein A in immunoperoxidase techniques.

Authors:  M R Celio; H Lutz; H Binz; H Fey
Journal:  J Histochem Cytochem       Date:  1979-02       Impact factor: 2.479

5.  Production and characterization of an antiserum to synthetic gonadotropin-releasing hormone.

Authors:  Y Koch; M Wilchek; M Fridkin; P Chobsieng; U Zor; H R Lindner
Journal:  Biochem Biophys Res Commun       Date:  1973-12-10       Impact factor: 3.575

6.  Ultrastructural localization of tyrosine hydroxylase in noradrenergic neurons of brain.

Authors:  V M Pickel; T H Joh; D J Reis
Journal:  Proc Natl Acad Sci U S A       Date:  1975-02       Impact factor: 11.205

7.  The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

Authors:  R C Graham; M J Karnovsky
Journal:  J Histochem Cytochem       Date:  1966-04       Impact factor: 2.479

8.  A formalin fixative for immunochemical and ultrastructural studies on gastrointestinal endocrine cells.

Authors:  G Robinson; I Dawson
Journal:  J Clin Pathol       Date:  1979-01       Impact factor: 3.411

9.  Immunocytochemical demonstration of anterior pituitary hormones in the pars tuberalis of long-term hypophysectomized rats.

Authors:  P Ordronneau; P Petrusz
Journal:  Am J Anat       Date:  1980-08

10.  Somatostatin-containing neurons in the rat brain: widespread distribution revealed by immunocytochemistry after pretreatment with pronase.

Authors:  J C Finley; G H Grossman; P Dimeo; P Petrusz
Journal:  Am J Anat       Date:  1978-11
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  28 in total

1.  Unfixed tissue for electron immunocytochemistry: a simple preparation method for colloidal gold localization of sensitive epitopes using ethanediol dehydration.

Authors:  J W Stirling
Journal:  Histochem J       Date:  1992-04

2.  Immunocytochemical localization of the gonadotropin-releasing hormone-associated peptide portion of the LHRH precursor in the hypothalamus and extrahypothalamic regions of the rat central nervous system.

Authors:  I Merchenthaler; M D Culler; P Petrusz; B Flerkó; A Negro-Vilar
Journal:  Cell Tissue Res       Date:  1989-01       Impact factor: 5.249

3.  Immunocytochemical staining of cytocentrifuge prepared cultured cells: nonspecific staining and its elimination.

Authors:  E J Gosselin; J C Dennett; G D Sorenson; O S Pettengill; C C Cate
Journal:  Histochem J       Date:  1985-08

4.  Increased sensitivity in immunocytochemistry. Effects of double application of antibodies and of silver intensification on immunogold and peroxidase-antiperoxidase staining techniques.

Authors:  L Scopsi; L I Larsson
Journal:  Histochemistry       Date:  1985

5.  Immunoassay and ultrastructural localization of isopentenyladenine and related cytokinins using monoclonal antibodies.

Authors:  J Eberle; T L Wang; S Cook; B Wells; E W Weiler
Journal:  Planta       Date:  1987-11       Impact factor: 4.116

6.  Temperature effect on immunostaining of microtubule-associated protein 2 and synaptophysin after 30 minutes of forebrain ischemia in rat.

Authors:  T Miyazawa; P Bonnekoh; K A Hossmann
Journal:  Acta Neuropathol       Date:  1993       Impact factor: 17.088

7.  Deficient activity of FAD-linked glycerophosphate dehydrogenase in islets of GK rats.

Authors:  C G Ostenson; S M Abdel-Halim; J Rasschaert; F Malaisse-Lagae; S Meuris; A Sener; S Efendic; W J Malaisse
Journal:  Diabetologia       Date:  1993-08       Impact factor: 10.122

8.  A new enzyme-linked immunosorbent assay (ELISA) for studying immunocytochemical procedures using an antiserum produced against spermidine as a model.

Authors:  K Fujiwara; M Araki; T Kitagawa; Y Inoue
Journal:  Histochemistry       Date:  1993-06

9.  Differentiation of cells producing polypeptide hormones (ACTH, MSH, LPH, alpha- and beta-endorphin, GH and PRL) in the fetal porcine anterior pituitary.

Authors:  F Dacheux
Journal:  Cell Tissue Res       Date:  1984       Impact factor: 5.249

10.  Immunohistochemical localization of carbonic anhydrase I and II in eccrine sweat glands from control subjects and patients with cystic fibrosis.

Authors:  J V Briggman; R E Tashian; S S Spicer
Journal:  Am J Pathol       Date:  1983-09       Impact factor: 4.307
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