Combination of cholinesterase staining of nerves and stereoscopic viewing for three-dimensional study of skin innervation on whole mounts.

A histochemical staining technique for cholinesterases is reported for the visualization of skin innervation on whole mounts in the chick. A method for realization of stereoscopic pictures showing the nerve fiber pattern in the full thickness of preparations is described. The combination of these 2 techniques allows an excellent three-dimensional demonstration of skin innervation.