Literature DB >> 7439188

The protochlorophyllide holochrome of barley (Hordeum vulgare L.). Isolation and characterization of the NADPH:protochlorophyllide oxidoreductase.

K Apel, H J Santel, T E Redlinger, H Falk.   

Abstract

The NADPH:protochlorophyllide oxidoreductase of barley has been solubilized from etioplast membranes and purified to apparent homogeneity. The highest specific activity measured for the purified enzyme was 1.6 nmol chlorophyllide formed (mg protein-1) per flash. Electrophoretic analysis of the purified enzyme on sodium dodecylsulfate/polyacrylamide gels revealed only one polypeptide of Mr 36000. Durig glycerol gradient centrifugation the enzyme migrates as a low-molecular-weight component. It is proposed that each enzyme molecule contains only one polypeptide chain. Assuming a molecular weight of 36000 for the enzyme, it was calculated that two or three protochlorophyllide molecules are bound to each enzyme molecule.

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Year:  1980        PMID： 7439188     DOI： 10.1111/j.1432-1033.1980.tb06100.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  59 in total

1.  A plastid enzyme arrested in the step of precursor translocation in vivo.

Authors:  S Reinbothe; C Reinbothe; D Neumann; K Apel
Journal:  Proc Natl Acad Sci U S A       Date:  1996-10-15       Impact factor: 11.205

Review 2.  Biogenesis of thylakoid networks in angiosperms: knowns and unknowns.

Authors:  Zach Adam; Dana Charuvi; Onie Tsabari; Ronit Rimon Knopf; Ziv Reich
Journal:  Plant Mol Biol       Date:  2010-09-22       Impact factor: 4.076

3.  ABI3 affects plastid differentiation in dark-grown Arabidopsis seedlings.

Authors:  A Rohde; R De Rycke; T Beeckman; G Engler; M Van Montagu; W Boerjan
Journal:  Plant Cell       Date:  2000-01       Impact factor: 11.277

4.  The effects of environmental light on the reorganization of chloroplasts in the resurrection of Selaginella tamariscina.

Authors:  Xinyu Li; Shuai Liu; Qiaojun Wang; Hongyang Wu; Yinglang Wan
Journal:  Plant Signal Behav       Date:  2019-05-25

5.  Molecular cloning, nuclear gene structure, and developmental expression of NADPH: protochlorophyllide oxidoreductase in pea (Pisum sativum L.).

Authors:  A J Spano; Z He; H Michel; D F Hunt; M P Timko
Journal:  Plant Mol Biol       Date:  1992-03       Impact factor: 4.076

6.  Chloroplast biogenesis: the use of mutants to study the etioplast-chloroplast transition.

Authors:  Katrin Philippar; Tina Geis; Iryna Ilkavets; Ulrike Oster; Serena Schwenkert; Jörg Meurer; Jürgen Soll
Journal:  Proc Natl Acad Sci U S A       Date:  2007-01-03       Impact factor: 11.205

7.  Novel Insights into the Enzymology, Regulation and Physiological Functions of Light-dependent Protochlorophyllide Oxidoreductase in Angiosperms.

Authors:  Tatsuru Masuda; Ken-Ichiro Takamiya
Journal:  Photosynth Res       Date:  2004       Impact factor: 3.573

Review 8.  Dose-dependent effects of 1O2 in chloroplasts are determined by its timing and localization of production.

Authors:  Liangsheng Wang; Klaus Apel
Journal:  J Exp Bot       Date:  2019-01-01       Impact factor: 6.992

9.  Chlorophyll Synthesis in a Deetiolated (det340) Mutant of Arabidopsis without NADPH-Protochlorophyllide (PChlide) Oxidoreductase (POR) A and Photoactive PChlide-F655.

Authors:  N. Lebedev; B. Van Cleve; G. Armstrong; K. Apel
Journal:  Plant Cell       Date:  1995-12       Impact factor: 11.277

10.  Two NADPH:Protochlorophyllide Oxidoreductases in Barley: Evidence for the Selective Disappearance of PORA during the Light-Induced Greening of Etiolated Seedlings.

Authors:  S. Reinbothe; C. Reinbothe; H. Holtorf; K. Apel
Journal:  Plant Cell       Date:  1995-11       Impact factor: 11.277
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