Literature DB >> 7438545

Quantitation of circulating immune complexes in serum by Raji cells using an enzyme-linked immunosorbent assay.

C Cunningham-Rundles, W E Brandeis, T Zacharczuk, R A Good, N K Day.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) for the quantitation of immune complexes in sera has been developed using Raji cells. In this assay the Raji cell-adherent complexes are detected by an alkaline phosphatase-conjugated rabbit anti-human IgG and the results are obtained by spectrophotometric measurements of the concentration of yellow colour produced after the enzyme's substrate, p-nitrophenyl phosphate, is added. This assay is as sensitive and reproducible as the Raji cell radioimmune assay using 125I, as it can detect as little as 16 micrograms of immune complex equivalent to heat-aggregated IgG/ml in serum samples. This method is inexpensive, convenient and most importantly, avoids the biohazards of using an isotope.

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Year:  1980        PMID： 7438545      PMCID： PMC1536970     

Source DB:  PubMed          Journal:  Clin Exp Immunol        ISSN： 0009-9104            Impact factor:   4.330


  10 in total

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Authors:  C G Cochrane; D Koffler
Journal:  Adv Immunol       Date:  1973       Impact factor: 3.543

2.  A method of trace iodination of proteins for immunologic studies.

Authors:  P J McConahey; F J Dixon
Journal:  Int Arch Allergy Appl Immunol       Date:  1966

3.  Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

Authors:  E Engvall; P Perlmann
Journal:  J Immunol       Date:  1972-07       Impact factor: 5.422

4.  Immune complex diseases.

Authors:  F J Dixon
Journal:  J Invest Dermatol       Date:  1972-12       Impact factor: 8.551

5.  Detection of antibodies and soluble antigen-antibody complexes by precipitation with polyethylene glycol.

Authors:  W D Creighton; P H Lambert; P A Miescher
Journal:  J Immunol       Date:  1973-10       Impact factor: 5.422

6.  Precipitin reactions of the C1q component of complement with aggregated gamma-globulin and immune complexes in gel diffusion.

Authors:  V Agnello; R J Winchester; H G Kunkel
Journal:  Immunology       Date:  1970-12       Impact factor: 7.397

7.  The Raji cell radioimmune assay for detecting immune complexes in human sera.

Authors:  A N Theofilopoulos; C B Wilson; F J Dixon
Journal:  J Clin Invest       Date:  1976-01       Impact factor: 14.808

8.  Circulating immune complexes in the serum in systemic lupus erythematosus and in carriers of hepatitis B antigen. Quantitation by binding to radiolabeled C1q.

Authors:  U E Nydegger; P H Lambert; H Gerber; P A Miescher
Journal:  J Clin Invest       Date:  1974-08       Impact factor: 14.808

9.  An unusual protein component of high molecular weight in the serum of certain patients with rheumatoid arthritis.

Authors:  E C FRANKLIN; H R HOLMAN; H J MULLER-EBERHARD; H G KUNKEL
Journal:  J Exp Med       Date:  1957-05-01       Impact factor: 14.307

10.  Immunological studies concerning the nephritis of systemic lupus erythematosus.

Authors:  D Koffler; P H Schur; H G Kunkel
Journal:  J Exp Med       Date:  1967-10-01       Impact factor: 14.307
  10 in total
  4 in total

1.  Acute and chronic immunological response to dietary antigen.

Authors:  P J Gallagher; N J Goulding; M J Gibney; D B Jones; J Morgan
Journal:  Gut       Date:  1983-09       Impact factor: 23.059

2.  Demonstration of IgG Fc receptors on spermatozoa and their utilization for the detection of circulating immune complexes in human serum.

Authors:  S S Witkin; S K Shahani; S Gupta; R A Good; N K Day
Journal:  Clin Exp Immunol       Date:  1980-09       Impact factor: 4.330

3.  Evaluation of an enzyme-linked immunosorbent Raji cell assay (ELISA) in the investigation of gastrointestinal cancer.

Authors:  S H Leveson; L F Woodhouse; G R Giles
Journal:  Cancer Immunol Immunother       Date:  1984       Impact factor: 6.968

4.  Peripheral nervous tissue injury induced by galactocerebroside and galactocerebroside immune complexes.

Authors:  N Tsukada; C S Koh; N Yanagisawa; T Taketomi; P O Behan
Journal:  Acta Neuropathol       Date:  1985       Impact factor: 17.088
  4 in total

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