| Literature DB >> 7436883 |
J G Hurrell, P H Black, E Haber, V R Zurawski.
Abstract
The selective removal of nonviable cells from suspension cultures of lymphoid cell lines by buoyant density centrifugation at 400 x g on a Ficoll-Hypaque (FH) cushion is described. Viable cells collect at the medium-FH interface whereas nonviable cells pellet. This method has proven useful in separating viable from nonviable cells with human lymphoblastoid cell lines, rabbit lymphoid cell lines and mouse myeloma and hybridoma cell lines.Entities:
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Year: 1980 PMID： 7436883 DOI： 10.1038/icb.1980.36
Source DB: PubMed Journal: Aust J Exp Biol Med Sci ISSN： 0004-945X