Literature DB >> 7433129

In vitro translation studies of the cytoplasmic nonpolysomal particles containing messenger RNA.

J P Liautard, J M Egly.   

Abstract

We analyzed the translational capacity of different kinds of free cytoplasmic messenger ribonucleoprotein complexes (free mRNP) in a Hela cell cell free system. Native free mRNP are not translated although free mRNP washed with 0.5 M KC1 can direct polypeptide synthesis. Furthermore, the 0.5 M KC1 wash possesses a factor which inhibits the translation of 0.5 M KC1 washed free mRNP as well as globin mRNA naked mRNA from plasmocytoma, or Hela cells. We also demonstrated that native free mRNP are able to form a complex with ribosomal subunits in the presence of initiation factors. This indicates that inhibition of translation by the 0.5 M KC1 wash occurs either at some point after initiation complex formation or at the elongation step.

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Year:  1980        PMID： 7433129      PMCID： PMC324036          DOI： 10.1093/nar/8.8.1793

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  22 in total

1.  Studies on a nonpolysomal ribonucleoprotein coding for myosin heavy chains from chick embryonic muscles.

Authors:  J Bag; S Sarkar
Journal:  J Biol Chem       Date:  1976-12-10       Impact factor: 5.157

2.  The cytoplasmic synthesis of histones in hela cells and its temporal relationship to DNA replication.

Authors:  E Robbins; T W Borun
Journal:  Proc Natl Acad Sci U S A       Date:  1967-02       Impact factor: 11.205

3.  Characterization of a protein kinase-phosphoprotein system in free cytoplasmic ribonucleoprotein particles of plasma cell tumours.

Authors:  J M Egly; M Schmitt; J Kempf
Journal:  Biochim Biophys Acta       Date:  1976-12-13

4.  Model for the regulation of mRNA translation applied to haemoglobin synthesis.

Authors:  H F Lodish
Journal:  Nature       Date:  1974-10-04       Impact factor: 49.962

5.  Separation of specific initiation factors involved in the translation of myosin and myoglobin messenger RNAs and the isolation of a new RNA involved in translation.

Authors:  S M Heywood; D S Kennedy; A J Bester
Journal:  Proc Natl Acad Sci U S A       Date:  1974-06       Impact factor: 11.205

6.  Preparation of globin messenger RNA.

Authors:  A W Nienhuis; A K Falvey; W F Anderson
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

7.  Isolation of cytoplasmic non-ribosomal ribonucleoprotein particles (informosomes).

Authors:  J Kempf; J M Egly; C Stricker; M Schmitt; P Mandel
Journal:  FEBS Lett       Date:  1972-10-01       Impact factor: 4.124

8.  Messenger RNA in HeLa cells: an investigation of free and polyribosome-bound cytoplasmic messenger ribonucleoprotein particles by kinetic labelling and electron microscopy.

Authors:  G Spohr; N Granboulan; C Morel; K Scherrer
Journal:  Eur J Biochem       Date:  1970-12

9.  Translation of maternal messenger ribonucleoprotein particles from sea urchin in a cell-free system from unfertilized eggs and product analysis.

Authors:  J Ilan; J Ilan
Journal:  Dev Biol       Date:  1978-10       Impact factor: 3.582

10.  Peptide coding capacity of polysomal and non-polysomal messenger RNA during growth of animal cells.

Authors:  G T Lee; D L Engelhardt
Journal:  J Mol Biol       Date:  1979-04-05       Impact factor: 5.469
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  8 in total

Review 1.  The function of proteins that interact with mRNA.

Authors:  D E Larson; B H Sells
Journal:  Mol Cell Biochem       Date:  1987-03       Impact factor: 3.396

2.  Potential role of mRNP proteins in cytoplasmic control of gene expression in duck erythroblasts.

Authors:  A Vincent; S Goldenberg; N Standart; O Civelli; T Imaizumi-Scherrer; K Maundrell; K Scherrer
Journal:  Mol Biol Rep       Date:  1981-05-22       Impact factor: 2.316

3.  Small cytoplasmic RNAs from rat liver mRNP particles. Studies on their structure and function.

Authors:  A Villringer; B Kühn; K Schneider; T Andus; K Michels; W Northemann; G Krupp; B Walckhof; H J Gross; H Klump; A Kroll; C Branlant; P C Heinrich
Journal:  Mol Biol Rep       Date:  1983-05       Impact factor: 2.316

4.  Cytoplasmic nonpolysomal ribonucleoprotein complexes and translational control.

Authors:  J Bag; B H Sells
Journal:  Mol Cell Biochem       Date:  1981-11-13       Impact factor: 3.396

5.  Cytoplasmic utilization of liposome-encapsulated myosin heavy chain messenger ribonucleoprotein particles. During muscle cell differentiation.

Authors:  A S Havaranis; S M Heywood
Journal:  Proc Natl Acad Sci U S A       Date:  1981-11       Impact factor: 11.205

6.  Cytoplasmic polyadenylate processing events accompany the transfer of mRNA from the free mRNP particles to the polysomes in Physarum.

Authors:  D S Adams; D Noonan; W R Jeffery
Journal:  Proc Natl Acad Sci U S A       Date:  1981-01       Impact factor: 11.205

7.  Differential repression of specific mRNA in erythroblast cytoplasm: a possible role for free mRNP proteins.

Authors:  A Vincent; O Akhayat; S Goldenberg; K Scherrer
Journal:  EMBO J       Date:  1983       Impact factor: 14.012

8.  Messenger RNA species partially in a repressed state in mouse sarcoma ascites cells.

Authors:  R Yenofsky; I Bergmann; G Brawerman
Journal:  Proc Natl Acad Sci U S A       Date:  1982-10       Impact factor: 12.779
  8 in total

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