Literature DB >> 743245

A rapid method for the preparation of relatively pure metabolically competent synaptosomes from rat brain.

R F Booth, J B Clark.   

Abstract

A rapid (less than 2h) method is described for the preparation of synaptosomes from rat brain by using a discontinuous Ficoll/sucrose gradient by a flotation technique. These synaptosomes are metabolically active and minimally (less than 5%) contaminated with 'free' mitochondria as judged by marker-enzyme assays and electron microscopy.

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Year:  1978        PMID： 743245      PMCID： PMC1186243          DOI： 10.1042/bj1760365

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  20 in total

1.  Synaptic and non-synaptic mitochondria from rat brain: isolation and characterization.

Authors:  J C Lai; J M Walsh; S C Dennis; J B Clark
Journal:  J Neurochem       Date:  1977-03       Impact factor: 5.372

2.  Cholinergic and non-cholinergic nerve endings in rat brain. I. Isolation and subcellular distribution of acetylcholine and acetylcholinesterase.

Authors:  E DE ROBERTIS; A PELLEGRINO DE IRALDI; G RODRIGUEZ DE LORES GARNAIZ; L SALGANICOFF
Journal:  J Neurochem       Date:  1962 Jan-Feb       Impact factor: 5.372

3.  The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation.

Authors:  E G GRAY; V P WHITTAKER
Journal:  J Anat       Date:  1962-01       Impact factor: 2.610

4.  The respiratory chain and oxidative phosphorylation.

Authors:  B CHANCE; G R WILLIAMS
Journal:  Adv Enzymol Relat Subj Biochem       Date:  1956

5.  Isolation and partial characterization of rat brain synaptic plasma membranes.

Authors:  J W Gurd; L R Jones; H R Mahler; W J Moore
Journal:  J Neurochem       Date:  1974-02       Impact factor: 5.372

6.  Cation modulation of synaptosomal respiration.

Authors:  M A Verity
Journal:  J Neurochem       Date:  1972-05       Impact factor: 5.372

7.  The metabolism of rat brain mitochondria. Preparation and characterization.

Authors:  J B Clark; W J Nicklas
Journal:  J Biol Chem       Date:  1970-09-25       Impact factor: 5.157

Review 8.  Synaptic macromolecules: identification and metabolism.

Authors:  S H Barondes
Journal:  Annu Rev Biochem       Date:  1974       Impact factor: 23.643

9.  Enzyme localization in the inner and outer membranes of rat liver mitochondria.

Authors:  D S Beattie
Journal:  Biochem Biophys Res Commun       Date:  1968-06-28       Impact factor: 3.575

10.  Electron transport systems of yeast. 3. Preparation and properties of cytochrome oxidase.

Authors:  H M Duncan; B Mackler
Journal:  J Biol Chem       Date:  1966-04-25       Impact factor: 5.157
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  92 in total

1.  Immunological identification of A2 adenosine receptors by two antipeptide antibody preparations.

Authors:  T M Palmer; K A Jacobson; G L Stiles
Journal:  Mol Pharmacol       Date:  1992-09       Impact factor: 4.436

2.  High-affinity transport of choline and amino acid neurotransmitters in synaptosomes from brain regions after lesioning the nucleus basalis magnocellularis of young and aged rats.

Authors:  J Gomeza; C Aragón; C Giménez
Journal:  Neurochem Res       Date:  1992-04       Impact factor: 3.996

3.  RNA splicing capability of live neuronal dendrites.

Authors:  J Glanzer; K Y Miyashiro; J-Y Sul; L Barrett; B Belt; P Haydon; J Eberwine
Journal:  Proc Natl Acad Sci U S A       Date:  2005-11-07       Impact factor: 11.205

4.  Regulation of cytosolic free calcium concentration by intrasynaptic mitochondria.

Authors:  A Martínez-Serrano; J Satrústegui
Journal:  Mol Biol Cell       Date:  1992-02       Impact factor: 4.138

5.  Comparison of the phosphate-dependent glutaminase obtained from rat brain and kidney.

Authors:  W G Haser; R A Shapiro; N P Curthoys
Journal:  Biochem J       Date:  1985-07-15       Impact factor: 3.857

6.  Calcium transport abnormality in uremic rat brain synaptosomes.

Authors:  C L Fraser; P Sarnacki; A I Arieff
Journal:  J Clin Invest       Date:  1985-11       Impact factor: 14.808

7.  Inhibition of adenylate cyclase in rat brain synaptosomal membranes by GTP and phenylisopropyladenosine is enhanced in hypothyroidism.

Authors:  D Mazurkiewicz; E D Saggerson
Journal:  Biochem J       Date:  1989-11-01       Impact factor: 3.857

8.  Lead as an inductor of some morphological and functional changes in synaptosomes from rat brain.

Authors:  L Jabłońska; M Walski; U Rafałowska
Journal:  Cell Mol Neurobiol       Date:  1994-12       Impact factor: 5.046

9.  Mechanism of depolarization of rat cortical synaptosomes at submicromolar external Ca2+ activity. The use of Ca2+ buffers to control the synaptosomal membrane potential.

Authors:  G Schmalzing
Journal:  Biochem J       Date:  1985-02-01       Impact factor: 3.857

10.  Micromolar-affinity benzodiazepine receptors regulate voltage-sensitive calcium channels in nerve terminal preparations.

Authors:  W C Taft; R J DeLorenzo
Journal:  Proc Natl Acad Sci U S A       Date:  1984-05       Impact factor: 11.205
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