Literature DB >> 7430655

Isolation of human NK cells by density gradient centrifugation.

T Timonen, E Saksela.   

Abstract

Sedimentation characteristics of human NK cells in discontinuous Percoll density gradients were studied. NK activity against the leukaemic cell line K-562 peaked in a single low density fraction mainly consisting of large granular lymphocytes previously shown to be the principal NK cells in human peripheral blood. Percoll density gradient centrifugation provides a useful tool for analysis of human NK cells and their relationship to other blood mononuclear cells.

Entities:  

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Year:  1980        PMID： 7430655     DOI： 10.1016/0022-1759(80)90133-7

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  83 in total

1.  A human immunoglobulin G receptor exists in both polypeptide-anchored and phosphatidylinositol-glycan-anchored forms.

Authors:  B J Scallon; E Scigliano; V H Freedman; M C Miedel; Y C Pan; J C Unkeless; J P Kochan
Journal:  Proc Natl Acad Sci U S A       Date:  1989-07       Impact factor: 11.205

2.  The effect of experimental haemocarbofiltration upon activity of mononuclear cells from normal and autoimmune patients.

Authors:  S A Grando; B T Glukhenky; G N Drannik; A P Kostromin; A B Romanenko
Journal:  Immunology       Date:  1989-01       Impact factor: 7.397

3.  Induction of the 2B9 antigen/dipeptidyl peptidase IV/CD26 on human natural killer cells by IL-2, IL-12 or IL-15.

Authors:  T Yamabe; K Takakura; K Sugie; Y Kitaoka; S Takeda; Y Okubo; K Teshigawara; J Yodoi; T Hori
Journal:  Immunology       Date:  1997-05       Impact factor: 7.397

4.  Functional surface structures on human natural killer cells.

Authors:  R E Schmidt; T Hercend; S F Schlossman; J Ritz
Journal:  Klin Wochenschr       Date:  1985-12-02

5.  Loss of natural killer activity as an indicator of relapse in acute leukaemia.

Authors:  J A Tratkiewicz; J Szer
Journal:  Clin Exp Immunol       Date:  1990-05       Impact factor: 4.330

6.  Human spontaneous lymphocyte-mediated cytotoxicity (SLMC) against malignant and normal tissue-derived target cell lines tested in autologous and allogeneic combinations by the microcytotoxicity assay.

Authors:  M Vilien; M Troye-Blomberg; P Perlmann; H Wolf; F Rasmussen
Journal:  Cancer Immunol Immunother       Date:  1983       Impact factor: 6.968

7.  Heterogeneous activity of immature and mature cells of the murine monocyte-macrophage lineage derived from different anatomical districts against yeast-phase Candida albicans.

Authors:  T Decker; M L Lohmann-Matthes; M Baccarini
Journal:  Infect Immun       Date:  1986-11       Impact factor: 3.441

8.  Natural killer cell and islet killer cell activities in type 1 (insulin-dependent) diabetes.

Authors:  K Negishi; N Waldeck; G Chandy; B Buckingham; A Kershnar; L Fisher; S Gupta; M A Charles
Journal:  Diabetologia       Date:  1986-06       Impact factor: 10.122

9.  Regulation of natural and antibody-dependent cellular cytotoxicity by staphylococcal enterotoxin A.

Authors:  I Kimber; T Bakács; M Moore
Journal:  Clin Exp Immunol       Date:  1983-10       Impact factor: 4.330

10.  The effect of the platelet-activating factor antagonist, BN 52021, on human natural killer cell-mediated cytotoxicity.

Authors:  Y Mandi; G Farkas; M Koltai; I Beladi; J M Mencia-Huerta; P Braquet
Journal:  Immunology       Date:  1989-07       Impact factor: 7.397
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