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Literature DB >> 7430085

ATP formation from adenyl-5'-yl imidodiphosphate, a nonhydrolyzable ATP analog.

Abstract

The purity of several preparations of adenyl-5'-yl imidodiphosphate (AMP-PNP) was analyzed using thin layer chromatography and the luciferin-luciferase assay. Three contaminants were identified: adenyl-5'-yl phosphoramidate, phosphorylated AMP-PNP, and ATP. The level of ATP contamination ranged from 0.02% to 0.3% in commercially obtained AMP-PNP preparations, and rose to 10% following incubation of AMP-PNP at 37 degrees C for 3 weeks in aqueous solution. The chemistry of the phosphoramidate bond is reviewed briefly, and evidence for a simple mechanism for the spontaneous formation of ATP from AMP-PNP is presented.

Entities: Chemical

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Year: 1980 PMID： 7430085

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157

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Cited

12 in total

1. The mitochondrial protein import motor: dissociation of mitochondrial hsp70 from its membrane anchor requires ATP binding rather than ATP hydrolysis.

Authors: M Horst; W Oppliger; B Feifel; G Schatz; B S Glick
Journal: Protein Sci Date: 1996-04 Impact factor: 6.725

2. The inhibition of muscle contraction by adenosine 5' (beta, gamma-imido) triphosphate and by pyrophosphate.

Authors: E Pate; R Cooke
Journal: Biophys J Date: 1985-06 Impact factor: 4.033

3. ATP dependence of vesicular stomatitis virus transcription initiation and modulation by mutation in the nucleocapsid protein.

Authors: J Perrault; P W McLear
Journal: J Virol Date: 1984-09 Impact factor: 5.103

7. The effect of the ATP analogue AMPPNP on the structure of crossbridges in vertebrate skeletal muscles: X-ray diffraction and mechanical studies.

Authors: R Padrón; H E Huxley
Journal: J Muscle Res Cell Motil Date: 1984-12 Impact factor: 2.698

ATP formation from adenyl-5'-yl imidodiphosphate, a nonhydrolyzable ATP analog.

1. The mitochondrial protein import motor: dissociation of mitochondrial hsp70 from its membrane anchor requires ATP binding rather than ATP hydrolysis.

2. The inhibition of muscle contraction by adenosine 5' (beta, gamma-imido) triphosphate and by pyrophosphate.

3. ATP dependence of vesicular stomatitis virus transcription initiation and modulation by mutation in the nucleocapsid protein.

4. Effect of the beta-gamma phosphate bond of ATP on synthesis of leader RNA and mRNAs of vesicular stomatitis virus.

5. Muscle cross-bridge kinetics in rigor and in the presence of ATP analogues.

6. An investigation into the role of ATP in the mammalian pre-mRNA 3' cleavage reaction.

7. The effect of the ATP analogue AMPPNP on the structure of crossbridges in vertebrate skeletal muscles: X-ray diffraction and mechanical studies.

8. Effects of AMPPNP and vanadate on the mechanochemical crossbridge cycle in flagella.

9. ADP binding to myosin cross-bridges and its effect on the cross-bridge detachment rate constants.

10. The motile beta/IC1 subunit of sea urchin sperm outer arm dynein does not form a rigor bond.