Literature DB >> 7428175

Improvements in the determination of urea using diacetyl monoxime; methods with and without deproteinisation.

M Rahmatullah, T R Boyde.   

Abstract

A rapid and reproducible method is described for measurement of urea in biological materials (after deproteinisation) and in serum (without deproteinisation). Urea is colorimetrically determined with diacetyl monoxime and thiosemicarbazide in the presence of sulphuric acid, phosphoric acid and ferric chloride. The sensitivity of the colorimetric reaction and stability of the colour are enhanced over existing related procedures and the serum blank diminished, enabling urea to be precisely measured in micro amounts (1--5 microliters) of serum.

Entities:  

Mesh：

Substances：

Year:  1980        PMID： 7428175     DOI： 10.1016/0009-8981(80)90407-6

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN： 0009-8981            Impact factor:   3.786


  50 in total

1.  Analysis of strains lacking known osmolyte accumulation mechanisms reveals contributions of osmolytes and transporters to protection against abiotic stress.

Authors:  Lindsay Murdock; Tangi Burke; Chelsea Coumoundouros; Doreen E Culham; Charles E Deutch; James Ellinger; Craig H Kerr; Samantha M Plater; Eric To; Geordie Wright; Janet M Wood
Journal:  Appl Environ Microbiol       Date:  2014-06-20       Impact factor: 4.792

2.  Widespread use of emersion and cutaneous ammonia excretion in Aplocheiloid killifishes.

Authors:  Michael D Livingston; Vikram V Bhargav; Andy J Turko; Jonathan M Wilson; Patricia A Wright
Journal:  Proc Biol Sci       Date:  2018-08-15       Impact factor: 5.349

3.  Mechanism of ammonia excretion in the freshwater leech Nephelopsis obscura: characterization of a primitive Rh protein and effects of high environmental ammonia.

Authors:  Alex R Quijada-Rodriguez; Jason R Treberg; Dirk Weihrauch
Journal:  Am J Physiol Regul Integr Comp Physiol       Date:  2015-07-15       Impact factor: 3.619

4.  Physiological protective action of dissolved organic carbon on ion regulation and nitrogenous waste excretion of zebrafish (Danio rerio) exposed to low pH in ion-poor water.

Authors:  Rafael M Duarte; Chris M Wood; Adalberto L Val; D Scott Smith
Journal:  J Comp Physiol B       Date:  2018-06-11       Impact factor: 2.200

5.  An in vitro study of urea and ammonia production and transport by the intestinal tract of fed and fasted rainbow trout: responses to luminal glutamine and ammonia loading.

Authors:  Ellen H Jung; Joanna Smich; Julian G Rubino; Chris M Wood
Journal:  J Comp Physiol B       Date:  2021-01-07       Impact factor: 2.200

6.  Prolonged survival and cytoplasmic pH homeostasis of Helicobacter pylori at pH 1.

Authors:  K Stingl; E M Uhlemann Em; G Deckers-Hebestreit; R Schmid; E P Bakker; K Altendorf
Journal:  Infect Immun       Date:  2001-02       Impact factor: 3.441

7.  Ammonia excretion in Caenorhabditis elegans: mechanism and evidence of ammonia transport of the Rhesus protein CeRhr-1.

Authors:  Aida Adlimoghaddam; Mélanie Boeckstaens; Anna-Maria Marini; Jason R Treberg; Ann-Karen C Brassinga; Dirk Weihrauch
Journal:  J Exp Biol       Date:  2015-03       Impact factor: 3.312

8.  Effect of dietary protein on intake, nutrients utilization, nitrogen balance, blood metabolites, growth and puberty in growing Bhadawari buffalo (Bubalus bubalis) heifers.

Authors:  Sultan Singh; Badri Prasad Kushwaha; Subendu Bikas Maity; Krishan Kunwar Singh; Nityanand Das
Journal:  Trop Anim Health Prod       Date:  2014-11-19       Impact factor: 1.559

9.  Antioxidant and Nephroprotective Activities of Aconitum heterophyllum Root in Glycerol Induced Acute Renal Failure in Rats.

Authors:  Venu Gopala Rao Konda; Madhavi Eerike; Lakshmipathy Prabhu Raghuraman; Maignana Kumar Rajamanickam
Journal:  J Clin Diagn Res       Date:  2016-03-01

10.  Ammonia and urea excretion in the tidepool sculpin (Oligocottus maculosus): sites of excretion, effects of reduced salinity and mechanisms of urea transport.

Authors:  P A Wright; P Part; C M Wood
Journal:  Fish Physiol Biochem       Date:  1995-04       Impact factor: 2.794
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.