| Literature DB >> 7419870 |
M M Krahn, D W Brown, T K Collier, A J Friedman, R G Jenkins, D C Malins.
Abstract
A rapid procedure for the determination of naphthalene and its metabolites in bile of rainbow trout and mice is described. The integrated analytical techniques combine high-performance liquid chromatography/ultraviolet fluorescence detection and plasma desorption/chemical ionization mass spectrometry for identification and quantitation. After separation by reverse-phase liquid chromatography, naphthalene and its metabolites are detected and quantitated by ultraviolet fluorescence spectrometry. Identification of two metabolites is confirmed by mass spectrometry. A direct insertion probe tip for a conventional chemical ionization mass spectrometer was modified to obtain spectra of thermally labile compounds. A spectrum of less than 100 ng of naphthyl glucuronide, a labile glucuronic acid conjugate of 1-naphthol, was obtained with this system.Entities:
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Year: 1980 PMID： 7419870 DOI： 10.1016/0165-022x(80)90038-x
Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X