| Literature DB >> 7419860 |
Abstract
A simple and sensitive automated method has been developed to separate monosaccharides by reverse-phase chromatography using the amino acid analyzer column in the Li+ form with 90% alcohol as eluent. The column can be regenerated with LiBr and 90% ethanol to achieve highly reproducible resolution of sugar components. Glycoproteins and polysaccharides are hydrolyzed by a combination of methanesulfonic acid/Dowex 50 X 8 resin in water. Glucose and mannose are distinguished by treatment of the hydrolysate with glucose oxidase prior to analysis.Entities:
Mesh：
Substances：
Year: 1980 PMID： 7419860 DOI： 10.1016/0165-022x(80)90075-5
Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X