Literature DB >> 7419234

Preparation of highly purified cytotrophoblast from human placenta with subsequent modulation to form syncytiotrophoblast in monolayer cultures.

C Cotte, G C Easty, A M Neville, P Monaghan.   

Abstract

The preparation of highly purified monolayer cultures of human cytotrophoblast cells essentially free of stromal and syncytial cells is described. Such cells subsequently form multinucleate syncytial cells in vitro. This is accompanied by the synthesis of heat-stable alkaline phosphatase and beta-HCG. A significant proportion of the multinucleate cells that form in monolayer cultures of early placentae arise as a result of an amitosis. It is suggested that this in vitro behavior may reflect an in vivo process.

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Year:  1980        PMID： 7419234     DOI： 10.1007/bf02619191

Source DB:  PubMed          Journal:  In Vitro        ISSN： 0073-5655


  18 in total

1.  THE ORIGIN AND FUNCTION OF HUMAN SYNCYTIOTROPHOBLASTIC GIANT CELLS.

Authors:  G B PIERCE; A R MIDGLEY
Journal:  Am J Pathol       Date:  1963-08       Impact factor: 4.307

2.  Studies of the human trophoblast in tissue culture. 1. Cultural methods and histochemical staining.

Authors:  H A THIEDE
Journal:  Am J Obstet Gynecol       Date:  1960-04       Impact factor: 8.661

3.  Effects of sodium butyrate on synthesis of human chorionic gonadotrophin in trophoblastic and non-trophoblastic tumours.

Authors:  J Y Chou; J C Robinson; S S Wang
Journal:  Nature       Date:  1977-08-11       Impact factor: 49.962

4.  The plasma membrane of human placenta. Isolation of microvillus membrane and characterization of protein and glycoprotein subunits.

Authors:  R W Carlson; H G Wada; H H Sussman
Journal:  J Biol Chem       Date:  1976-07-10       Impact factor: 5.157

5.  Viability of human trophoblast in vitro.

Authors:  P V Taylor; K W Hancock
Journal:  J Obstet Gynaecol Br Commonw       Date:  1973-09

6.  Trophoblast giant cell differentiation in the mouse: expression of glucose phosphate isomerase (GPI-1) electrophoretic variants in transferred and chimeric embryos.

Authors:  V M Chapman; J D Ansell; A McLaren
Journal:  Dev Biol       Date:  1972-09       Impact factor: 3.582

7.  Glucosephosphate isomerase subunit-reassociation tests for maternal-fetal and fetal-fetal cell fusion in the mouse placenta.

Authors:  J D Gearhart; B Mintz
Journal:  Dev Biol       Date:  1972-09       Impact factor: 3.582

8.  [Spectrophotometric determination of the degree of polyploidy of decidual cells in the endometrium of white rats].

Authors:  E V Zybina; T A Grishchenko
Journal:  Tsitologiia       Date:  1972-03

9.  Use of polyethylene glycol to separate free and antibody-bound peptide hormones in radioimmunoassays.

Authors:  B Desbuquois; G D Aurbach
Journal:  J Clin Endocrinol Metab       Date:  1971-11       Impact factor: 5.958

10.  Short term culture of human midterm and term placenta: parameters of hormonogenesis.

Authors:  C S Hall; T E James; C Goodyer; C Branchaud; H Guyda; C J Giroud
Journal:  Steroids       Date:  1977-10       Impact factor: 2.668
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  10 in total

1.  Use of differentiating human trophoblasts in in vitro assay.

Authors:  M Jaggi; S Farheen; P K Mehrotra
Journal:  In Vitro Cell Dev Biol Anim       Date:  1999-09       Impact factor: 2.416

2.  Identification of human chorionic gonadotropin (HCG) secreting cells and other cell types using antibody to HCG and a new monoclonal antibody (mABlu-5) in cultures of human placental villi.

Authors:  M Frauli; H Ludwig
Journal:  Arch Gynecol Obstet       Date:  1987       Impact factor: 2.344

3.  Viability of the human placental villi in organ culture.

Authors:  M Ogino; M Satoh; K Kinoshita; K Satoh; M Mizuno; S Sakamoto
Journal:  Arch Gynecol       Date:  1984

4.  Human placental cytotrophoblast cells: identification and culture.

Authors:  P Truman; L Pomare; H C Ford
Journal:  Arch Gynecol Obstet       Date:  1989       Impact factor: 2.344

5.  Extracellular vesicles generated by placental tissues ex vivo: A transport system for immune mediators and growth factors.

Authors:  Wendy Fitzgerald; Nardhy Gomez-Lopez; Offer Erez; Roberto Romero; Leonid Margolis
Journal:  Am J Reprod Immunol       Date:  2018-05-04       Impact factor: 3.886

6.  Spontaneous cell transformation: karyoplasts derived from multinucleated cells produce new cell growth in senescent human epithelial cell cultures.

Authors:  Kirsten H Walen
Journal:  In Vitro Cell Dev Biol Anim       Date:  2004 May-Jun       Impact factor: 2.416

7.  Characterization of hCG regulation in cultured human amniotic fluid cells: II. Mechanisms for stimulation.

Authors:  C H Laundon; J H Priest; R E Priest
Journal:  In Vitro       Date:  1983-12

8.  The effect of substrate and epidermal growth factor on human placental trophoblast cells in culture.

Authors:  P Truman; H C Ford
Journal:  In Vitro Cell Dev Biol       Date:  1986-09

9.  Adhesive and degradative properties of human placental cytotrophoblast cells in vitro.

Authors:  S J Fisher; T Y Cui; L Zhang; L Hartman; K Grahl; G Y Zhang; J Tarpey; C H Damsky
Journal:  J Cell Biol       Date:  1989-08       Impact factor: 10.539

10.  Transcriptional control of HLA-A,B,C antigen in human placental cytotrophoblast isolated using trophoblast- and HLA-specific monoclonal antibodies and the fluorescence-activated cell sorter.

Authors:  M Kawata; J R Parnes; L A Herzenberg
Journal:  J Exp Med       Date:  1984-09-01       Impact factor: 14.307
  10 in total

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